Ocular Surface Involvements in the Development of Sjögren's Syndrome-Associated Dry Eye in the IL14α Transgenic Mouse.

IF 5 2区 医学 Q1 OPHTHALMOLOGY
Minjie Zhang, Yichen Liang, Han Wu, Rongrong Zong, Xiaobo Zhang, Hui He, Peter Sol Reinach, Zuguo Liu, Long Shen, Wei Li
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引用次数: 0

Abstract

Purpose: To investigate the ocular surface changes during progress of the Sjögren's Syndrome (SS), using a previously described IL14α transgenic mice (IL14α TG) SS model.

Methods: The ocular surface of IL14α TG and C57BL/6 wild-type (WT) female mice were evaluated at the age of six, nine, 12, 15, and 18 months. Slit lamp microscopy observation, Oregon green dextran staining, Schirmer test, and periodic-acid-Schiff staining were assessed. Immunohistochemistry, immunofluorescence, and associated gene expression analysis by qPCR and ELISA were performed in cornea, conjunctiva, and lacrimal grand at different ages of the mice. Masson's trichome staining was conducted on lacrimal gland cryosections.

Results: Compared with C57BL/6 WT mice, IL14α TG mice showed corneal barrier function damage and losses in conjunctival goblet cell density starting at nine months, whereas decreases in tear secretion started at 18 months of age. Significant increases in CD4+ T cell infiltration in the conjunctiva of IL14α TG mice was first observed at 6 months. Higher expression levels of inflammatory cytokines IL-17A, IFN-γ, IL-1β, and TNF-α in the conjunctiva, whereas MUC5AC and MUC5B had lower expression levels at nine months in the IL14α TG mice. However, lacrimal gland function-associated gene expression levels mostly decreased in IL14α TG mice at 12 months of age.

Conclusions: Ocular surface tissue changes were involved in SS-like dry eye in a time-dependent manner in IL14α TG mice, and conjunctival T-cell infiltration may contribute to ocular surface pathological changes in an early stage of SS-related dry eye.

il - 14α转基因小鼠Sjögren综合征相关性干眼的眼表参与
目的:利用先前描述的IL14α转基因小鼠(IL14α TG) SS模型研究Sjögren's综合征(SS)进展过程中的眼表变化。方法:分别在6、9、12、15、18月龄时观察il - 14α TG和C57BL/6野生型(WT)雌性小鼠的眼表。观察裂隙灯显微镜观察、俄勒冈绿葡聚糖染色、Schirmer试验和周期性酸-席夫染色。采用免疫组化、免疫荧光及qPCR和ELISA方法对不同年龄小鼠角膜、结膜和泪腺进行相关基因表达分析。泪腺冷冻切片进行马氏毛状染色。结果:与C57BL/6 WT小鼠相比,IL14α TG小鼠在9月龄时出现角膜屏障功能损伤,结膜杯状细胞密度下降,泪液分泌减少。在6个月时首次观察到IL14α TG小鼠结膜内CD4+ T细胞浸润显著增加。炎症因子IL-17A、IFN-γ、IL-1β和TNF-α在结膜中的表达水平较高,而MUC5AC和MUC5B在9个月时在IL14α TG小鼠中表达水平较低。然而,在12月龄时,泪腺功能相关基因表达水平在IL14α TG小鼠中大部分下降。结论:il - 14α - TG小鼠眼表组织改变以时间依赖性的方式参与ss样干眼,结膜t细胞浸润可能参与ss相关性干眼早期眼表病理改变。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
CiteScore
6.90
自引率
4.50%
发文量
339
审稿时长
1 months
期刊介绍: Investigative Ophthalmology & Visual Science (IOVS), published as ready online, is a peer-reviewed academic journal of the Association for Research in Vision and Ophthalmology (ARVO). IOVS features original research, mostly pertaining to clinical and laboratory ophthalmology and vision research in general.
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