Investigating the modulatory effects of Pu-erh tea on the gut microbiota in ameliorating hyperuricemia induced by circadian rhythm disruption†

IF 5.1 1区 农林科学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY
Food & Function Pub Date : 2025-02-19 DOI:10.1039/D4FO05659K
Sibo Zhao, Hongli Cao, Fanwei Sun, Mianhong Xu, Xinghua Wang, Jielin Jiang, Liyong Luo and Liang Zeng
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引用次数: 0

Abstract

Circadian rhythm disruption (CRD) can induce a variety of metabolic disorders. Our previous laboratory studies have shown that Pu-erh tea could alleviate CRD-induced syndromes, including obesity, intestinal dysfunction, and tryptophan metabolism disorders. However, its potential protective mechanism against CRD-induced hyperuricaemia remains unclear. In this work, we found that polyphenols of Pu-erh tea were significantly released in the stage of intestinal digestion, which might promote their interaction with gut microbes. Through animal experiments, C57BL6/J mice were given water or different doses of Pu-erh tea for 60 days, followed by a 90-day CRD, the lifestyle of modern individuals who frequently stay up late. Our results indicated that CRD mice exhibited high serum uric acid levels and gut microbiota disorders. Pu-erh tea intake significantly reshaped the gut microbiome, especially increasing the abundance of Bifidobacterium, Akkermansia and Faecalibaculum, and increased the production of short-chain fatty acids (SCFAs), especially acetic acid, which restored the function of the intestinal barrier. This improvement further regulated oxidative stress pathways (NRF2/HO-1), reduced systemic inflammatory response (IL-6, IL-1β, and TNF-α), restored hepatic function (SOD, MOD, CAT, and GSH) and modulated the activity of enzymes related to UA metabolism in the liver (XOD and ADA). Finally, Pu-erh tea intake promoted the excretion of UA and reduced the levels of UA and xanthine in the serum. Moreover, the results of antibiotic experiments showed that the UA improvement effect of Pu-erh tea depended on the existence of the gut microbiota. Collectively, Pu-erh tea intake has the potential to prevent CRD-induced hyperuricaemia by reshaping the gut microbiota.

Abstract Image

昼夜节律紊乱(CRD)可诱发多种代谢紊乱。我们之前的实验室研究表明,普洱茶可以缓解昼夜节律紊乱诱发的综合征,包括肥胖、肠道功能紊乱和色氨酸代谢紊乱。然而,普洱茶对 CRD 引起的高尿酸血症的潜在保护机制仍不清楚。在这项工作中,我们发现普洱茶中的茶多酚在肠道消化阶段被大量释放,这可能会促进它们与肠道微生物的相互作用。通过动物实验,给 C57BL6/J 小鼠喂水或不同剂量的普洱茶 60 天,然后进行 90 天的 CRD(现代人经常熬夜的生活方式)。我们的研究结果表明,CRD小鼠的血清尿酸水平较高,肠道微生物群紊乱。摄入普洱茶能明显重塑肠道微生物群,特别是增加双歧杆菌、Akkermansia和Faecalibaculum的丰度,并增加短链脂肪酸(SCFAs),尤其是乙酸的产生,从而恢复肠道屏障的功能。这种改善进一步调节了氧化应激途径(NRF2/HO-1),降低了全身炎症反应(IL-6、IL-1β 和 TNF-α),恢复了肝功能(SOD、MOD、CAT 和 GSH),并调节了肝脏中与 UA 代谢有关的酶的活性(XOD 和 ADA)。最后,普洱茶能促进尿酸的排泄,降低血清中尿酸和黄嘌呤的水平。此外,抗生素实验结果表明,普洱茶对尿酸的改善作用取决于肠道微生物群的存在。总之,普洱茶可通过重塑肠道微生物群来预防 CRD 引起的高尿酸血症。
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来源期刊
Food & Function
Food & Function BIOCHEMISTRY & MOLECULAR BIOLOGY-FOOD SCIENCE & TECHNOLOGY
CiteScore
10.10
自引率
6.60%
发文量
957
审稿时长
1.8 months
期刊介绍: Food & Function provides a unique venue for physicists, chemists, biochemists, nutritionists and other food scientists to publish work at the interface of the chemistry, physics and biology of food. The journal focuses on food and the functions of food in relation to health.
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