Identification of VHA genes related to organic acid metabolism and functional analysis of key genes in Cerasus humilis

IF 3.9 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-02-15 DOI:10.1016/j.scienta.2025.114034

Shuai Zhang , Jie Zhang , Lu Zhang , HaoJia Ye , ChenYi Wang , Xiaopeng Mu , Jiancheng Zhang , LuTing Jia , Yu Gary Gao , Pengfei Wang

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引用次数: 0

Abstract

The content and types of organic acids contribute significantly to the varietal differences in the quality of Cerasus humilis fruits. The molecular mechanisms underlying the accumulation of organic acids in C. humilis remain poorly understood. The study first identified the role of VHA family genes in malic acid metabolism in the low-acid variety '3–19–3′ and the high-acid variety 'Jinou 1′, identifying the VHA genes ChVHA-V0-a3 and ChVHA-V1-G3 that may be related to malic acid metabolism. Furthermore, we conducted extensive validations of the candidate VHA genes in mature fruit of Cerasus humilis 45 accessions, as well as during the fruit development of the low-acid variety '3–6–8′ and the high-acid variety 'Ziou 1′. Finally, the transient transformation technology was utilized to verify the functions of the candidate VHA genes. The results from this study provided new insights into the molecular mechanisms behind the regulation of malic acid production in C. humilis fruits.

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.