A widespread plant defense compound disarms bacterial type III injectisome assembly

IF 44.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Pub Date : 2025-02-28

Pei Miao, Haijun Wang, Wei Wang, Zhengdong Wang, Han Ke, Hangyuan Cheng, Jinjing Ni, Jingnan Liang, Yu-Feng Yao, Jizong Wang, Jian-Min Zhou, Xiaoguang Lei

引用次数: 0

Abstract

Numerous gram-negative bacterial pathogens employ the type III secretion system (T3SS), a multiprotein injectisome, to deliver virulence proteins into host cells and cause diseases. We uncover erucamide as a previously unknown phytoalexin of both dicots and monocots that blocks the T3SS function of multiple bacterial pathogens. Genetically impairing erucamide accumulation or exogenous application in Arabidopsis highlighted erucamide’s role in antibacterial immunity. Erucamide binds Hypersensitive response and conserved C (HrcC), a key T3SS component, to block injectisome assembly. Analyses of erucamide analogs and HrcC mutants indicated that the erucamide-HrcC binding is required for inhibiting T3SS in vitro and antibacterial resistance in plants, suggesting an essential role of erucamide-HrcC binding in disease resistance. This work reveals a plant chemical defense that targets major virulence machinery in bacterial pathogens.

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许多革兰氏阴性细菌病原体利用 III 型分泌系统（T3SS）--一种多蛋白注射体--将毒力蛋白输送到宿主细胞中并致病。我们发现芥酰胺是双子叶植物和单子叶植物中一种以前未知的植物毒素，它能阻断多种细菌病原体的 T3SS 功能。在拟南芥中通过基因影响芥酰胺的积累或外源应用凸显了芥酰胺在抗菌免疫中的作用。芥酰胺能与超敏反应和保守 C（HrcC）结合，后者是 T3SS 的关键组成部分，能阻止注射体的组装。对芥酰胺类似物和 HrcC 突变体的分析表明，芥酰胺与 HrcC 的结合是体外抑制 T3SS 和植物抗菌所必需的，这表明芥酰胺与 HrcC 的结合在抗病中起着至关重要的作用。这项研究揭示了一种针对细菌病原体主要毒力机制的植物化学防御方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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