Guoguo Jin, Man Zhang , Xinran Wang, Yifan Zhang, Guohua Jiang, Lin Mei
{"title":"Characteristics of exopolysaccharides - egg white protein composite gel and its application in low - fat sausage","authors":"Guoguo Jin, Man Zhang , Xinran Wang, Yifan Zhang, Guohua Jiang, Lin Mei","doi":"10.1016/j.fochx.2025.102290","DOIUrl":null,"url":null,"abstract":"<div><div>A composite gel was developed by integrating antioxidant extracellular polysaccharides (EPS) derived from <em>Pediococcus acidilactici</em> S1 with egg white protein (EWP), aiming to evaluate its potential as a viable alternative to animal fat in pork sausages. The results indicated that the EPS - EWP gel exhibited a lower free water content, an enhanced water - holding capacity, a higher apparent viscosity, and increased storage and loss modulus. Molecular interactions were strengthened, resulting in a more stable structure characterized by the transition of secondary structure from random coils to ordered β - sheets. Molecular docking (MD) analysis revealed favorable binding conformations and strong binding energies between ovalbumin (OVA) and EPS, particularly through the formation of specific pockets involving interactions with residues such as Lysine (Lys) and Aspartic acid (Asp). Hydrophobic and electrostatic forces were identified as the primary driving forces for this energetic combination. Additionally, low - fat sausages showed a significant 32.87 % improvement in inhibiting fat oxidation.</div></div>","PeriodicalId":12334,"journal":{"name":"Food Chemistry: X","volume":"26 ","pages":"Article 102290"},"PeriodicalIF":6.5000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry: X","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2590157525001373","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0
Abstract
A composite gel was developed by integrating antioxidant extracellular polysaccharides (EPS) derived from Pediococcus acidilactici S1 with egg white protein (EWP), aiming to evaluate its potential as a viable alternative to animal fat in pork sausages. The results indicated that the EPS - EWP gel exhibited a lower free water content, an enhanced water - holding capacity, a higher apparent viscosity, and increased storage and loss modulus. Molecular interactions were strengthened, resulting in a more stable structure characterized by the transition of secondary structure from random coils to ordered β - sheets. Molecular docking (MD) analysis revealed favorable binding conformations and strong binding energies between ovalbumin (OVA) and EPS, particularly through the formation of specific pockets involving interactions with residues such as Lysine (Lys) and Aspartic acid (Asp). Hydrophobic and electrostatic forces were identified as the primary driving forces for this energetic combination. Additionally, low - fat sausages showed a significant 32.87 % improvement in inhibiting fat oxidation.
期刊介绍:
Food Chemistry: X, one of three Open Access companion journals to Food Chemistry, follows the same aims, scope, and peer-review process. It focuses on papers advancing food and biochemistry or analytical methods, prioritizing research novelty. Manuscript evaluation considers novelty, scientific rigor, field advancement, and reader interest. Excluded are studies on food molecular sciences or disease cure/prevention. Topics include food component chemistry, bioactives, processing effects, additives, contaminants, and analytical methods. The journal welcome Analytical Papers addressing food microbiology, sensory aspects, and more, emphasizing new methods with robust validation and applicability to diverse foods or regions.