Naringin vs. Citrus x paradisi L. Peel Extract: An In Vivo Journey into Oxidative Stress Modulation.

IF 6 2区医学 Q1 BIOCHEMISTRY & MOLECULAR BIOLOGY

Antioxidants Pub Date : 2025-01-28 DOI:10.3390/antiox14020157

Jolita Stabrauskiene, Ilona Sadauskiene, Arunas Liekis, Zoja Mikniene, Jurga Bernatoniene

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Abstract

Citrus fruits, mainly grapefruit (Citrus x paradisi L.), are rich in bioactive compounds with potential antioxidant properties. This study investigated the antioxidant effects of naringin (NR) and ethanolic Citrus x paradisi L. peel (E) in reducing aluminum chloride (AlCl₃)-induced oxidative stress in mice. Quantitative analysis using HPLC identified optimal extraction conditions, combination ultrasound and reflux extraction (UH50), resulting in high concentrations of naringin (49.13 mg/g) and naringenin (63.99 µg/g). Mice were treated with NR and E to evaluate their effects on key markers of oxidative stress: reduced glutathione (GSH), malondialdehyde (MDA), and catalase (CAT). The E effectively reduced MDA levels in blood, brain, and liver tissues, with a more substantial effect on controlling lipid peroxidation. In contrast, NR was more effective in restoring GSH levels and CAT activity, suggesting a broader enhancement of antioxidant defense. These findings provide information about specific mechanisms of NR and E and their therapeutic potential in managing oxidative stress and developing products with synergistic efficacy.

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柚皮苷与柑桔皮提取物：体内氧化应激调节之旅。

柑橘类水果，主要是葡萄柚（Citrus x paradisi L.），富含具有潜在抗氧化特性的生物活性化合物。研究柚皮苷（naringin， NR）和乙醇天门冬皮（Citrus x paradisi L. peel， E）对氯化铝（AlCl3）诱导的小鼠氧化应激的抗氧化作用。定量分析采用高效液相色谱法确定最佳提取条件，超声与回流联合提取（UH50），得到高浓度柚皮苷（49.13 mg/g）和柚皮苷（63.99µg/g）。用NR和E处理小鼠，以评估其对氧化应激关键标志物：还原型谷胱甘肽（GSH）、丙二醛（MDA）和过氧化氢酶（CAT）的影响。E能有效降低血液、脑和肝组织中的MDA水平，在控制脂质过氧化方面效果更显著。相反，NR在恢复GSH水平和CAT活性方面更有效，表明抗氧化防御有更广泛的增强作用。这些发现为NR和E的具体机制及其在控制氧化应激和开发具有协同功效的产品方面的治疗潜力提供了信息。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Antioxidants Biochemistry, Genetics and Molecular Biology-Physiology

CiteScore

10.60

自引率

11.40%

发文量

2123

审稿时长

16.3 days

期刊介绍： Antioxidants (ISSN 2076-3921), provides an advanced forum for studies related to the science and technology of antioxidants. It publishes research papers, reviews and communications. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. The full experimental details must be provided so that the results can be reproduced. Electronic files and software regarding the full details of the calculation or experimental procedure, if unable to be published in a normal way, can be deposited as supplementary electronic material.