Simultaneous determination of fumonisin B₁ and B₂ in maize using a facile online pre-column derivatization HPLC method.

IF 2.3 3区农林科学 Q2 CHEMISTRY, APPLIED

Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment Pub Date : 2025-04-01 Epub Date: 2025-02-25 DOI:10.1080/19440049.2025.2468793

Nan Li, Haodong Liu, Xinyue Hu, Lingmei Niu, Yijing Zhai, Xiangdong Xu

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引用次数: 0

Abstract

A rapid, sensitive and reliable analytical method for the simultaneous determination of fumonisin B₁ (FB₁) and fumonisin B₂ (FB₂) was developed and optimised using high performance liquid chromatography and fluorescence detection (HPLC-FLD) coupled with online pre-column derivatization using o-phthalaldehyde (OPA) and 2-mercaptoethanol (2-ME). This study focused on the optimisation of the online pre-column derivatization procedure, HPLC parameters, and sample pre-treatment. FB₁ and FB₂ in maize were extracted and purified using immunoaffinity column (IAC) and derived with OPA at room temperature. The OPA reagent was stable for 7 days, and OPA-derivatised products for 15 min. The excitation and emission wavelengths were 335 nm and 440 nm, respectively. Chromatography was performed using a C18 column and gradient elution at 1.0 mL min^-1 with acetonitrile and 0.1% acetic acid at pH 3.21. The LOD values of FB₁ and FB₂ were 0.10 and 0.26 mg kg^-1, respectively, comparable with or lower than those in other reports. The recoveries of FB₁ and FB₂ were between 85.6%-119.2%. The presented method is time-saving and robust, and successfully applied in determining FB₁ and FB₂ in maize.

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来源期刊

Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment CHEMISTRY, APPLIED-FOOD SCIENCE & TECHNOLOGY

CiteScore

7.40

自引率

6.90%

发文量

136

审稿时长

3 months

期刊介绍： Food Additives & Contaminants: Part A publishes original research papers and critical reviews covering analytical methodology, occurrence, persistence, safety evaluation, detoxification and regulatory control of natural and man-made additives and contaminants in the food and animal feed chain. Papers are published in the areas of food additives including flavourings, pesticide and veterinary drug residues, environmental contaminants, plant toxins, mycotoxins, marine biotoxins, trace elements, migration from food packaging, food process contaminants, adulteration, authenticity and allergenicity of foods. Papers are published on animal feed where residues and contaminants can give rise to food safety concerns. Contributions cover chemistry, biochemistry and bioavailability of these substances, factors affecting levels during production, processing, packaging and storage; the development of novel foods and processes; exposure and risk assessment.