Simultaneous determination of fumonisin B₁ and B₂ in maize using a facile online pre-column derivatization HPLC method.

IF 2.3 3区农林科学 Q2 CHEMISTRY, APPLIED

Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment Pub Date : 2025-04-01 Epub Date: 2025-02-25 DOI:10.1080/19440049.2025.2468793

Nan Li, Haodong Liu, Xinyue Hu, Lingmei Niu, Yijing Zhai, Xiangdong Xu

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引用次数: 0

Abstract

A rapid, sensitive and reliable analytical method for the simultaneous determination of fumonisin B₁ (FB₁) and fumonisin B₂ (FB₂) was developed and optimised using high performance liquid chromatography and fluorescence detection (HPLC-FLD) coupled with online pre-column derivatization using o-phthalaldehyde (OPA) and 2-mercaptoethanol (2-ME). This study focused on the optimisation of the online pre-column derivatization procedure, HPLC parameters, and sample pre-treatment. FB₁ and FB₂ in maize were extracted and purified using immunoaffinity column (IAC) and derived with OPA at room temperature. The OPA reagent was stable for 7 days, and OPA-derivatised products for 15 min. The excitation and emission wavelengths were 335 nm and 440 nm, respectively. Chromatography was performed using a C18 column and gradient elution at 1.0 mL min^-1 with acetonitrile and 0.1% acetic acid at pH 3.21. The LOD values of FB₁ and FB₂ were 0.10 and 0.26 mg kg^-1, respectively, comparable with or lower than those in other reports. The recoveries of FB₁ and FB₂ were between 85.6%-119.2%. The presented method is time-saving and robust, and successfully applied in determining FB₁ and FB₂ in maize.

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快速在线柱前衍生化高效液相色谱法同时测定玉米中伏马菌素B1和B2。

建立了一种快速、灵敏、可靠的同时测定伏马菌素B1 （FB1）和伏马菌素B2 （FB2）的高效液相色谱-荧光检测(HPLC-FLD) -邻苯二醛（OPA）和2-巯基乙醇（2-ME）在线衍生的分析方法。本研究的重点是在线柱前衍生化程序、HPLC参数和样品前处理的优化。采用免疫亲和柱（IAC）对玉米中FB1和FB2进行了提取纯化，室温下用OPA进行了衍生。OPA试剂稳定7天，OPA衍生物稳定15分钟。激发波长为335 nm，发射波长为440 nm。色谱柱为C18，梯度洗脱1.0 mL min-1，乙腈和0.1%醋酸，pH为3.21。FB1和FB2的LOD值分别为0.10和0.26 mg kg-1，与其他报道相当或低于。FB1和FB2的加样回收率在85.6% ~ 119.2%之间。该方法具有省时、鲁棒性好等优点，已成功应用于玉米中FB1和FB2的测定。

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来源期刊

Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment CHEMISTRY, APPLIED-FOOD SCIENCE & TECHNOLOGY

CiteScore

7.40

自引率

6.90%

发文量

136

审稿时长

3 months

期刊介绍： Food Additives & Contaminants: Part A publishes original research papers and critical reviews covering analytical methodology, occurrence, persistence, safety evaluation, detoxification and regulatory control of natural and man-made additives and contaminants in the food and animal feed chain. Papers are published in the areas of food additives including flavourings, pesticide and veterinary drug residues, environmental contaminants, plant toxins, mycotoxins, marine biotoxins, trace elements, migration from food packaging, food process contaminants, adulteration, authenticity and allergenicity of foods. Papers are published on animal feed where residues and contaminants can give rise to food safety concerns. Contributions cover chemistry, biochemistry and bioavailability of these substances, factors affecting levels during production, processing, packaging and storage; the development of novel foods and processes; exposure and risk assessment.