Hypoglycemic mechanism of foxtail millet prolamin hydrolysate in type 2 diabetic mice revealed by metabolomics and 16S rRNA sequencing

IF 4.8 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience Pub Date : 2025-02-21 DOI:10.1016/j.fbio.2025.106187

Yiqing Zhu , Tong Wu , Yongxia Fu , Qingyu Zhao , Li Yue , Liangxing Zhao , Li Zhi , Xiaomeng Wu , Yong Xue , Qun Shen

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引用次数: 0

Abstract

Foxtail millet prolamin hydrolysate (FMPH) has demonstrated hypoglycemic effects, though its underlying mechanisms remain unclear. This study used 16S rRNA sequencing and serum metabolomics to investigate the effects of FMPH on blood glucose metabolism in type 2 diabetes (T2D) mice (male, C57BL/6J). The results showed that FMPH supplementation improved weight loss, hyperglycemia, hyperlipidemia, insulin resistance, serum hormone levels, and inflammatory markers while mitigating liver, kidney, and pancreatic damage in T2D mice. FMPH shifted gut microbiota composition, increasing the abundance of Firmicutes and Actinomycetota while reducing Bacteroidota, which was associated with elevated concentrations of short-chain fatty acids, particularly acetic and propionic acids. Furthermore, FMPH normalized serum metabolites related to glycerophospholipid and tryptophan metabolism. Key metabolites, including PC(18:0/0:0) and PE(20:0/20:3(5Z,8Z,11Z)), were closely associated with specific bacteria (Ileibacterium and Bifidobacterium) and T2D indicators. These findings recommend that the hypoglycemic effects of FMPH may be mediated through gut microbiota alteration and metabolic regulation, highlighting its potential as a functional food for the prevention and management of T2D.

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来源期刊

Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

6.40

自引率

5.80%

发文量

671

审稿时长

27 days

期刊介绍： Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.