A lateral flow assay based on aptamer for the detection of AFM1 in milk samples

IF 4.8 1区农林科学 Q1 FOOD SCIENCE & TECHNOLOGY

Food Bioscience Pub Date : 2025-02-18 DOI:10.1016/j.fbio.2025.106175

Yiyao Wu , Mingwei Qin , Peifang Chen , Yu Li , Xiaodong Lu , Anqi Sun , Zhouping Wang , Junsong Yang

{"title":"A lateral flow assay based on aptamer for the detection of AFM1 in milk samples","authors":"Yiyao Wu , Mingwei Qin , Peifang Chen , Yu Li , Xiaodong Lu , Anqi Sun , Zhouping Wang , Junsong Yang","doi":"10.1016/j.fbio.2025.106175","DOIUrl":null,"url":null,"abstract":"<div><div>With the growth of the economy in recent years, the issue of food safety has gotten worse. Aflatoxin M<sub>1</sub> (AFM<sub>1</sub>) is a highly toxic substance that poses a serious health risk and is widely distributed in milk and other dairy products. Biosensors are widely used in the field of food safety, but there is a lack of on-site methods to detect AFM<sub>1</sub>. In this study, we propose for the first time an Apt-LFA based on AuNPs that can accurately detect AFM<sub>1</sub>. The test strip's linear range was 0–500 ng/mL, and its detection limit was as low as 0.21 ng/mL. The test strip's recoveries in actual milk samples reached 92%–104.35% with excellent accuracy and stability. The results of this study have important practical application value, filling the gap of aptamer paper-based sensor technology in the field of AFM<sub>1</sub> detection and expanding the application scope of aptamers in AFM<sub>1</sub> detection. The test strips can be used for point-of-care testing (POCT). It is an important contribution to the development of food safety.</div></div>","PeriodicalId":12409,"journal":{"name":"Food Bioscience","volume":"66 ","pages":"Article 106175"},"PeriodicalIF":4.8000,"publicationDate":"2025-02-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Bioscience","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S2212429225003517","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"FOOD SCIENCE & TECHNOLOGY","Score":null,"Total":0}

引用次数: 0

Abstract

With the growth of the economy in recent years, the issue of food safety has gotten worse. Aflatoxin M₁ (AFM₁) is a highly toxic substance that poses a serious health risk and is widely distributed in milk and other dairy products. Biosensors are widely used in the field of food safety, but there is a lack of on-site methods to detect AFM₁. In this study, we propose for the first time an Apt-LFA based on AuNPs that can accurately detect AFM₁. The test strip's linear range was 0–500 ng/mL, and its detection limit was as low as 0.21 ng/mL. The test strip's recoveries in actual milk samples reached 92%–104.35% with excellent accuracy and stability. The results of this study have important practical application value, filling the gap of aptamer paper-based sensor technology in the field of AFM₁ detection and expanding the application scope of aptamers in AFM₁ detection. The test strips can be used for point-of-care testing (POCT). It is an important contribution to the development of food safety.

查看原文本刊更多论文

求助全文

约1分钟内获得全文求助全文

来源期刊

Food Bioscience Biochemistry, Genetics and Molecular Biology-Biochemistry

CiteScore

6.40

自引率

5.80%

发文量

671

审稿时长

27 days

期刊介绍： Food Bioscience is a peer-reviewed journal that aims to provide a forum for recent developments in the field of bio-related food research. The journal focuses on both fundamental and applied research worldwide, with special attention to ethnic and cultural aspects of food bioresearch.