Study on the Allergenicity of Tropomyosin from Different Aquatic Products Based on Conformational and Linear Epitopes Analysis

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-02-13 DOI:10.1021/acs.jafc.4c1185310.1021/acs.jafc.4c11853

Liangtao Lv, Fangling Wei, Lu Liu, Futeng Song, Xiudan Hou and Qingli Yang*,

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Abstract

Tropomyosin (TM) is a major allergen in aquatic products. The aim of this study was to analyze the allergenicity of TM from different aquatic products based on conformational and linear epitopes. Structural and allergenicity analyses of TM were conducted using intrinsic fluorescence, UV absorption spectra, circular dichroism, and animal experiments. Epitope mapping was performed through bioinformatics software and a one-bead, one-compound (OBOC) peptide library screening approach. The results showed that the structures of TMs from different aquatic products are similar. Cross-reactivity was observed among TMs from different aquatic products, with fish-TM showing lower cross-reactivity compared with other TMs. Additionally, 13, 14, 11, 13, and 12 linear epitopes, along with 2, 2, 1, 2, and 3 conformational epitopes, were identified for shrimp-TM, crab-TM, fish-TM, oyster-TM, and clam-TM, respectively. Overall, these findings provide a basis for elucidating the epitope localization and allergenicity relationship of TMs from different aquatic products.

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基于构象和线性表位分析的不同水产品原肌球蛋白致敏性研究

原肌球蛋白（tromyosin， TM）是水产品中的主要过敏原。本研究的目的是基于构象表位和线性表位分析不同水产品TM的致敏性。采用本征荧光、紫外吸收光谱、圆二色性和动物实验对TM进行结构和致敏性分析。表位定位通过生物信息学软件和一头一化合物（OBOC）肽库筛选方法进行。结果表明，不同水产品中TMs的结构相似。不同水产品的tm具有交叉反应性，其中鱼tm具有较低的交叉反应性。此外，在虾- tm、蟹- tm、鱼- tm、牡蛎- tm和蛤- tm中分别鉴定出13、14、11、13和12个线性表位以及2、2、1、2和3个构象表位。总之，这些发现为阐明不同水产品中TMs的表位定位和致敏性关系提供了基础。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.