Grapevine rootstock genotypes shape the functional composition of phyllosphere microbiome

IF 3.9 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-02-15 DOI:10.1016/j.scienta.2025.114023

Sihai Zhang , Mengmeng Liu , Lefeng Lin , Yi Shen , Xuejun Pan , Ruipu Wang , Xianghua Fang , Xiangfeng Tan

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Abstract

The impact of rootstock selection and grafting on grapevines has been extensively studied, yet the influence on microbial functions in plant-associated environments remains underexplored. This study investigates the impact of rootstock genotypes on the grapevine microbiome in both the below-ground rhizosphere and the above-ground phyllosphere using metagenomic sequencing. Rootstock genotypes significantly influence the diversity and composition of prokaryotic taxa in rhizosphere soil. Conversely, shotgun sequencing reveals that rootstock genotypes have no impact on the taxonomic composition of the phyllosphere microbiome, although the functional gene composition does vary. In total, 252 microbial genes across 31 biogeochemical pathways were identified from the metagenomic sequences of the phyllosphere microbiome. Notably, phosphorus-cycling pathways involving purine and pyruvate metabolism showed distinct compositions among grapevine rootstock genotypes. The 25 microbial genes involved in purine and pyruvate metabolism were attributed to 482 microbial genera, with four archaeal and four Firmicutes genera each harboring no fewer than 10 genes. Additionally, the read counts of purine metabolism were significantly associated with leaf nitrogen and phosphorus contents. Our findings highlight the importance of rootstock selection in shaping the microbial functional composition in the grapevine phyllosphere, underscoring potential avenues for precision viticulture strategies that leverage microbiome management.

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.