Purification, characterization, and functional validation of a novel casein complex enzyme hydrolysate-binding calcium

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-02-16 DOI:10.1016/j.foodchem.2025.143438

Xin Wang , Xianwei Yuan , Ruyu Yan, Jianchen Song, Chuan Ren, Hongbo Li, Hongjuan Li, Jinghua Yu

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引用次数: 0

Abstract

Food Peptide Calcium Chelate was an excellent calcium supplement. The aim of this study was to isolate peptides with high calcium binding activity from a mixture of casein hydrolyzed peptides, to determine their structural characteristics and to verify their function. Firstly, micellar casein was hydrolyzed by a combination of flavor protease and trypsin. Casein hydrolysate peptides (CHP) with high calcium chelating activity were obtained by three purifications and characterized by high-performance liquid chromatography tandem mass spectrometry (HPLC-MS/MS), mass spectrometry (MS/MS), and fourier transform infrared spectroscopy (FTIR). The results showed that the purified polypeptide (Tyr-Gln-Glu-Pro) had high calcium binding capacity (70.10 ± 4.23 μg/mg). Animal experiments confirmed that YQEP-Ca was effective in improving the bone microarchitecture of rats, and that the low-calcium-content's medium-dose group also had better utilization than the inorganic and unchelated calcium groups. Therefore, the YQEP-Ca obtained in this study provides new clues for the development of various products.

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食物肽钙螯合物是一种很好的钙补充剂。本研究旨在从酪蛋白水解肽混合物中分离出具有高钙结合活性的肽，确定其结构特征并验证其功能。首先，用风味蛋白酶和胰蛋白酶组合水解胶束酪蛋白。通过三次纯化获得了具有高钙螯合活性的酪蛋白水解肽（CHP），并通过高效液相色谱串联质谱（HPLC-MS/MS）、质谱（MS/MS）和傅立叶变换红外光谱（FTIR）对其进行了表征。结果表明，纯化的多肽（Tyr-Gln-Glu-Pro）具有很高的钙结合能力（70.10 ± 4.23 μg/mg）。动物实验证实，YQEP-Ca 能有效改善大鼠的骨微结构，而且低钙含量的中剂量组的利用率也优于无机钙和无螯合钙组。因此，本研究获得的 YQEP-Ca 为各种产品的开发提供了新的线索。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.