In Vitro Cholesterol-Lowering Bioactivity, Synthetic Pathway, and Structural Characterization of Exopolysaccharide Synthesized by Schleiferilactobacillus harbinensis Z171

IF 6.2 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-01-16 DOI:10.1021/acs.jafc.4c0991610.1021/acs.jafc.4c09916

Jinsong Wu, Xianbo Cheng, Ziyi Wu, Sashuang Dong and Qingping Zhong*,

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Abstract

A strain identified as Schleiferilactobacillus harbinensis was isolated from Chinese sauerkraut, and its exopolysaccharide (EPS) exhibited excellent in vitro cholesterol-lowering bioactivity. Besides, the whole genome of this strain and the structure characteristics of the purified EPS were investigated in this study. S. harbinensis Z171 presented a strong EPS production capacity, with five nucleotide sugar biosynthesis pathways regulated by an EPS synthesis gene cluster. Structural characterization revealed that the purified fraction F-EPS1A was a neutral polysaccharide with a molecular weight of 6.4 × 10⁴ Da. The structure of F-EPS1A contained a backbone that comprised blocks of four 1,2-linked and three 1,3-linked alpha mannose units. Some 1,2-linked alpha mannose residues were branched at C6 with side chains formed by single alpha mannose or a disaccharide consisting of 1,6-linked alpha mannose residues. The structural characteristics endowed F-EPS1A with a high level of cholesterol-lowering bioactivity. In addition, whole genome analysis indicated that S. harbinensis Z171 possessed a strong EPS production capacity. These findings suggested that the EPS produced by S. harbinensis Z171 could be applied as a potential cholesterol-lowering prebiotic agent or supplement in functional food.

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哈宾施莱乳杆菌Z171合成的胞外多糖体外降胆固醇活性、合成途径及结构表征

从中国酸菜中分离到一株哈氏施莱乳杆菌，其胞外多糖（EPS）具有良好的体外降胆固醇活性。此外，本研究还对该菌株的全基因组和纯化EPS的结构特征进行了研究。海南S. harbinensis Z171具有较强的EPS生产能力，具有5条由EPS合成基因簇调控的核苷酸糖生物合成途径。结构表征表明纯化后的F-EPS1A为中性多糖，分子量为6.4 × 104 Da。F-EPS1A的结构包含一个由四个1,2-链和三个1,3-链α甘露糖单元组成的主链。一些1,2连接的α -甘露糖残基在C6上支链，侧链由单个α -甘露糖或由1,6连接的α -甘露糖残基组成的双糖形成。这种结构特征赋予F-EPS1A高水平的降胆固醇生物活性。此外，全基因组分析结果表明，海南S. harbinensis Z171具有较强的EPS生产能力。上述结果提示，哈滨河芽孢杆菌Z171产生的EPS可作为一种潜在的降胆固醇益生元制剂或功能性食品的补充剂。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.