Bioinspired synthesis of copper oxide nanoparticles using aqueous extracts of Cladophora glomerata (L.) Kuetz and their potential biomedical applications.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Bioprocess and Biosystems Engineering Pub Date : 2025-04-01 Epub Date: 2025-02-10 DOI:10.1007/s00449-025-03133-5

Vinay Kumar, Yadvinder Singh, Sandeep Kaushal, Ranvijay Kumar

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Abstract

Present study deals with the green fabrication of copper oxide nanoparticles (CuO NPs) employing cell-free aqueous extract of Cladophora glomerata (L.) Kuetz, freshwater algal species. The UV-visible, FTIR, XRD, FESEM, HRTEM, EDX, BET, XPS and Raman spectroscopic techniques were used to confirm and characterize the biosynthesized CuO NPs. The UV-Vis analysis revealed a sharp peak at 264 nm with a band gap of 3.7 eV, which was attributable to the fabrication of CuO NPs. FESEM and HRTEM detect the spherical-shaped morphology with size between 40 and 50 nm. The biochemical profiling of cell free extract of the C. glomerata by Gas chromatography-mass spectrometry (GC-MS) revealed the presence of various bioactive biomolecules that may acts as a precursor for the fabrication of CuO NPs. The antibacterial study of fabricated CuO NPs revealed significant growth inhibitory potential against selected bacterial strains Klebsiella pneumoniae and Bacillus cereus with an IC₅₀ value of 10 μg/ml. The synthesized CuO NPs also displayed strong DPPH radical scavenging (IC₅₀ value 11.25 mg/L) and total antioxidant (IC₅₀ value 11 mg/L) properties. Further, the anticancer activity of fabricated CuO NPs was studied employing a human hepatocellular carcinoma (HepG2) cell line by MTT assay, which marks their ability to diminish the 50% cell with IC₅₀ value of 168.6 µg/ml. Overall, the findings confirmed that CuO NPs fabricated employing cell-free extract of C. glomerata have the potential to be used as active agent in various biomedical applications after further detailed clinical investigations.

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利用鸭茅（Cladophora glomerata， L.）水提液生物合成氧化铜纳米颗粒Kuetz及其潜在的生物医学应用。

本研究主要研究了利用鸭茅（Cladophora glomerata， L.）无细胞水提物绿色制备氧化铜纳米粒子（CuO NPs）。Kuetz，淡水藻类。采用uv -可见、FTIR、XRD、FESEM、HRTEM、EDX、BET、XPS和拉曼光谱等技术对合成的CuO纳米粒子进行了表征。UV-Vis分析显示，在264 nm处有一个尖峰，带隙为3.7 eV，这是由于制备了CuO NPs。FESEM和HRTEM检测到尺寸在40 ~ 50 nm之间的球形形貌。利用气相色谱-质谱联用技术（GC-MS）对肾小球无细胞提取物进行生化分析，发现其含有多种生物活性分子，可能是制备CuO纳米粒子的前体。结果表明，制备的氧化铜NPs对肺炎克雷伯菌和蜡样芽孢杆菌有明显的生长抑制作用，IC50值为10 μg/ml。合成的CuO NPs具有较强的DPPH自由基清除能力（IC50值为11.25 mg/L）和总抗氧化能力（IC50值为11 mg/L）。在人肝癌（HepG2）细胞株上，用MTT法研究了制备的CuO NPs的抗癌活性，其IC50值为168.6µg/ml，表明其具有抑制50%细胞的能力。综上所述，经过进一步的临床研究，证实了利用无细胞提取物制备的CuO NPs具有在各种生物医学应用中作为活性药物的潜力。

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来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.