Structural Characterization of APSN from Astragalus membranaceus and Its Potential Therapeutic Effect on Immune Dysregulation and Tissue Damage

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-02-07 DOI:10.1021/acs.jafc.4c08632

Liqiu Hu, Qili Sun, Zhanpeng Liu, Hanwei Huang, Enze Zhao, Haotian Chen, Yuxun Wu, Yongmei Ge, Dongfang Ouyang, Bin Tang

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Abstract

Addressing the global health impact of inflammatory and immune-mediated diseases, this study focused on purifying and characterizing a neutral polysaccharide, APSN, from the Astragalus membranaceus. Employing high-performance gel permeation chromatography (HPGPC), Fourier-transform infrared spectroscopy (FTIR), and nuclear magnetic resonance (NMR) spectroscopy, we elucidated APSN’s structural features, revealing a highly branched glucan with a 1,4-α-d-glucopyranosyl main chain and side chains at the O-6 position. Separately, we assessed APSN’s biological activity, finding that it significantly modulates immune responses by inhibiting the NF-κB pathway in RAW264.7 macrophages and promotes endothelial cell proliferation and angiogenesis-related gene expression in HUVECs. These results position APSN as a potential therapeutic for diseases characterized by immune dysregulation and tissue damage, warranting further investigation of its mechanisms and clinical efficacy.

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.