Faraday cage-type photocurrent polarity switching photoelectrochemical sensing platform for highly selective and sensitive detection of Vibrio parahaemolyticus

IF 9.8 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-02-07 DOI:10.1016/j.foodchem.2025.143275

Qingqing Zhang , Tingting Zhai , Yuxin Guo , Yifan Weng , Nan Zhou , Han Lin , Hao Tan , Kangning Lu , Yiyi Zhou

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引用次数: 0

Abstract

Highly selective and sensitive detection of foodborne pathogens is crucial for ensuring food safety. In this work, a photoelectrochemical (PEC) aptasensing platform was developed for the selective and sensitive detection of Vibrio parahaemolyticus (VP), adopting Faraday cage-type construction mode. When VP was present in the sample, the aptamer for VP (Apt) assembled on the Apt/Bi₂S₃/ITO electrode captured VP, which bound GO@Cu₂O-Apt to the electrode surface. The graphene oxide (GO) in GO@Cu₂O-Apt has a large surface area and good conductivity, on the surface of which a large amount of Cu₂O that can switch the polarity of the Bi₂S₃ photocurrent was loaded, resulting in a highly selective and sensitive detection of VP with a detection limit of 1.0 CFU/mL. By adjusting the Apt, the platform can be used for the detection of other foodborne pathogens, offering broad application potential in foodborne pathogen detection.

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法拉第笼型光电流极性开关电化学传感平台高选择性、高灵敏度检测副溶血性弧菌

高度选择性和灵敏的食源性病原体检测对确保食品安全至关重要。本文采用法拉第笼型构建模式，建立了副溶血性弧菌（VP）的光电化学（PEC）适体感应平台，用于副溶血性弧菌（VP）的选择性和敏感性检测。当样品中存在VP时，组装在Apt/Bi2S3/ITO电极上的VP适配体（Apt）捕获VP，并将其结合GO@Cu2O-Apt到电极表面。GO@Cu2O-Apt中的氧化石墨烯（GO）具有较大的表面积和良好的导电性，在其表面加载了大量可以切换Bi2S3光电流极性的Cu2O，从而对VP具有高选择性和灵敏度，检测限为1.0 CFU/mL。通过调整Apt，该平台可用于其他食源性病原体的检测，在食源性病原体检测中具有广阔的应用潜力。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.