Sleep-enhancing activity of fermented pea protein hydrolysate with enhanced GABA content by Lactobacillus brevis SYLB 0016 fermentation

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-02-06 DOI:10.1002/jsfa.14173

Hyeon Deok Kim, Hyung Joo Suh, Seok Hyun Chung, Jongwon Byun, Yesol Yoo, Hyowon Lee

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引用次数: 0

Abstract

BACKGROUND

Sleep is essential for overall health and wellbeing. This study investigated the sleep-promoting effects of fermented pea protein hydrolysate (PPF) with increased gamma-aminobutyric acid (GABA) content produced by Lactobacillus brevis SYLB 0016. The effects of PPF on sleep duration and structure were assessed in pentobarbital-induced ICR mice and Sprague Dawley (SD) rats using electroencephalogram (EEG) analysis.

RESULTS

Hydrolysis of pea protein with Alcalase, Protana Prime, and Protana UBoost increased the amino nitrogen content, degree of hydrolysis and glutamate content to 160.51 mmol L⁻¹. Fermentation by Lactobacillus brevis SYLB 0016 increased the GABA content from 3.16 to 90.35 mmol L⁻¹. PPF significantly increased sleep duration (56.3 min) compared to the normal control (30.6 min) in pentobarbital-induced sleep tests. Non-rapid eye movement (NREM) sleep time increased with a significant rise in δ-waves activity following administration of 150 mg kg⁻¹ of PPF. In caffeine-induced insomnia, both low- and high-dose PPF significantly increased sleep duration. Three weeks of oral PPF administration elevated GABA_A and GABA_B receptor expression, with GABA_A receptor protein levels showing a significant change. Co-administration of flumazenil with PPF reduced sleep time, indicating the involvement of the GABA_A receptor benzodiazepine site in PPF's sleep-enhancing effects.

CONCLUSION

In conclusion, PPF with enhanced GABA content improves NREM sleep by increasing δ waves activity. As a hypoallergenic compound, PPF holds potential as a supplement to ameliorate sleep disorders. © 2025 Society of Chemical Industry.

查看原文本刊更多论文

短乳杆菌SYLB 0016发酵提高GABA含量的发酵豌豆蛋白水解物的促睡眠活性。

背景：睡眠对整体健康和幸福至关重要。本研究探讨了由短乳杆菌SYLB 0016产生的增加γ -氨基丁酸（GABA）含量的发酵豌豆蛋白水解物（PPF）的促睡眠作用。采用脑电图（EEG）分析PPF对戊巴比妥诱导的ICR小鼠和SD大鼠睡眠时间和结构的影响。结果：Alcalase、Protana Prime和Protana UBoost对豌豆蛋白的水解使其氨基氮含量、水解度和谷氨酸含量达到160.51 mmol L-1。短乳杆菌SYLB 0016发酵使GABA含量由3.16提高到90.35 mmol L-1。在戊巴比妥诱导睡眠试验中，与正常对照组（30.6分钟）相比，PPF显著增加了睡眠持续时间（56.3分钟）。给药150 mg kg-1 PPF后，非快速眼动（NREM）睡眠时间增加，δ波活动显著增加。在咖啡因引起的失眠中，低剂量和高剂量PPF都显著增加了睡眠时间。口服PPF 3周后，GABAA和GABAB受体表达升高，GABAA受体蛋白水平发生显著变化。氟马西尼与PPF联合使用减少了睡眠时间，表明GABAA受体苯二氮卓位点参与了PPF增强睡眠的作用。结论：GABA含量增加的PPF通过增加δ波活动改善NREM睡眠。作为一种低过敏性化合物，PPF有可能作为一种改善睡眠障碍的补充剂。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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