Assessing the in vitro efficiency in adsorbing mycotoxins of a tri-octahedral bentonite with potential application in aquaculture feed.

IF 2.3 3区农林科学 Q2 CHEMISTRY, APPLIED

Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment Pub Date : 2025-02-04 DOI:10.1080/19440049.2025.2459234

Xenia Pascari, Irene Teixido-Orries, Francisco Molino, Sonia Marin, Antonio J Ramos

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Abstract

The use of mycotoxin binders in feed products is currently the most efficient method to mitigate the harmful effects of mycotoxins. The unprecedented growth of aquaculture in recent years has led to an increased use of plant-based ingredients in fish feeds, thereby raising the risk of mycotoxin exposure. This study investigates the in vitro adsorption efficiency of a tri-octahedral bentonite against aflatoxin B₁ (AFB1), zearalenone (ZEN), and fumonisin B₁ (FB1) in simulated gastric (pH = 1.2) and intestinal (pH = 6.8) fluids at 25 °C, the usual body temperature in aquaculture fish species. The binder was highly effective, removing over 98% of AFB1 from both media. FB1 was completely adsorbed at pH = 1.2, while its adsorption at pH = 6.8 reached a maximum of 46.3%. ZEN binding was consistent across both pH levels, ranging from 56.1% to 69.7%. Nine equilibrium isotherm functions were fitted to the experimental data to elucidate the adsorption mechanisms. A Sips model isotherm best characterized AFB1 adsorption in simulated gastric fluid, whereas that of ZEN was best described by the Freundlich model. In simulated intestinal fluid (pH = 6.8), monolayer adsorption described by the Langmuir model provided the best fit for all three mycotoxins.

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评估一种可用于水产养殖饲料的三八面体膨润土对霉菌毒素的体外吸附效率。

目前，在饲料产品中使用霉菌毒素粘合剂是减轻霉菌毒素有害影响的最有效方法。近年来，随着水产养殖业的空前发展，鱼饲料中植物性成分的使用越来越多，从而增加了接触霉菌毒素的风险。本研究调查了三八面体膨润土对黄曲霉毒素 B1 (AFB1)、玉米赤霉烯酮 (ZEN) 和伏马菌素 B1 (FB1) 的体外吸附效率，吸附液为模拟胃液（pH = 1.2）和肠液（pH = 6.8），吸附温度为 25 °C（水产养殖鱼类通常的体温）。这种粘合剂非常有效，能从两种介质中去除 98% 以上的 AFB1。FB1 在 pH = 1.2 时被完全吸附，而在 pH = 6.8 时的吸附率最高，达到 46.3%。ZEN 在两个 pH 值水平上的结合率一致，从 56.1% 到 69.7% 不等。实验数据拟合了九种平衡等温线函数，以阐明吸附机制。在模拟胃液中，Sips 模型等温线最能说明 AFB1 的吸附特性，而 Freundlich 模型则最能说明 ZEN 的吸附特性。在模拟肠液（pH=6.8）中，Langmuir 模型描述的单层吸附对所有三种霉菌毒素都最适合。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Food Additives and Contaminants Part A-chemistry Analysis Control Exposure & Risk Assessment CHEMISTRY, APPLIED-FOOD SCIENCE & TECHNOLOGY

CiteScore

7.40

自引率

6.90%

发文量

136

审稿时长

3 months

期刊介绍： Food Additives & Contaminants: Part A publishes original research papers and critical reviews covering analytical methodology, occurrence, persistence, safety evaluation, detoxification and regulatory control of natural and man-made additives and contaminants in the food and animal feed chain. Papers are published in the areas of food additives including flavourings, pesticide and veterinary drug residues, environmental contaminants, plant toxins, mycotoxins, marine biotoxins, trace elements, migration from food packaging, food process contaminants, adulteration, authenticity and allergenicity of foods. Papers are published on animal feed where residues and contaminants can give rise to food safety concerns. Contributions cover chemistry, biochemistry and bioavailability of these substances, factors affecting levels during production, processing, packaging and storage; the development of novel foods and processes; exposure and risk assessment.