Nanofiltration-based purification process for whole-cell transformed prebiotic galactooligosaccharides.

IF 3.5 3区生物学 Q2 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Bioprocess and Biosystems Engineering Pub Date : 2025-04-01 Epub Date: 2025-02-04 DOI:10.1007/s00449-025-03132-6

Anita Srivastava, Arjun Rastogi, Avijeet S Jaswal, Jatindra K Sahu, Gopal P Agarwal, Saroj Mishra

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Abstract

The enrichment of galactooligosaccharides (GOS), synthesized by whole cells of Kluyveromyces marxianus 3551 in a 5.0-L bioreactor, was investigated in this study. The synthesized sugar mixture containing 17.89% (w/w of total carbohydrates) of GOS with 15.57% (w/w of total carbohydrates) of lactose, and 66.54% (w/w of total carbohydrates) monosaccharides as impurities, was subjected to nanofiltration for enrichment of GOS. Three distinct spiral wound membranes, namely, NFPS-01(polysulfone), NFCA-02 (cellulose acetate), and NFPES-03 (polyethersulfone) were tested out of which the NFPES-03 performed the best for fractionation of the GOS mixture. The polyethersulphone membrane (cut-off 400-1000 Da) was evaluated at 30 ℃ and 50 ℃, at different transmembrane pressures or TMP (15, 20, 25 bar) and a combination of high temperature (50 ℃) and low pressure (15 bar) gave the greatest difference in the trisaccharide and disaccharide/monosaccharide rejection percentages, resulting in enrichment of GOS. An analysis of the sugar concentrations in the retentate samples by high-performance liquid chromatography indicated the percentage recovery of GOS in the integrated process to be 88.8%. Measurement of the growth profile of several microbes on the nano-filtered GOS demonstrated its effectiveness as a prebiotic source.

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基于纳滤的全细胞转化益生元低聚半乳糖纯化工艺。

以马氏克鲁维菌3551全细胞为原料，在5.0 l生物反应器中进行了半乳糖寡糖（galactooligosaccharides， GOS）的富集研究。合成的糖混合物中含有17.89% （w/w）的GOS， 15.57% （w/w）的乳糖和66.54% （w/w）的单糖作为杂质，对GOS进行纳滤富集。对NFPS-01（聚砜）、NFCA-02（醋酸纤维素）和nfps -03（聚醚砜）三种不同的螺旋缠绕膜进行了测试，其中nfps -03对GOS混合物的分选效果最好。在30℃和50℃、不同跨膜压力或TMP（15、20、25 bar）以及高温（50℃）和低压（15 bar）组合条件下，聚醚砜膜（截止400-1000 Da）的三糖和双糖/单糖排斥率差异最大，导致GOS富集。采用高效液相色谱法对保留液中糖的浓度进行分析，结果表明，综合工艺中GOS的回收率为88.8%。对几种微生物在纳米过滤GOS上的生长曲线的测量表明其作为益生元来源的有效性。

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来源期刊

Bioprocess and Biosystems Engineering 工程技术-工程：化工

CiteScore

7.90

自引率

2.60%

发文量

147

审稿时长

2.6 months

期刊介绍： Bioprocess and Biosystems Engineering provides an international peer-reviewed forum to facilitate the discussion between engineering and biological science to find efficient solutions in the development and improvement of bioprocesses. The aim of the journal is to focus more attention on the multidisciplinary approaches for integrative bioprocess design. Of special interest are the rational manipulation of biosystems through metabolic engineering techniques to provide new biocatalysts as well as the model based design of bioprocesses (up-stream processing, bioreactor operation and downstream processing) that will lead to new and sustainable production processes. Contributions are targeted at new approaches for rational and evolutive design of cellular systems by taking into account the environment and constraints of technical production processes, integration of recombinant technology and process design, as well as new hybrid intersections such as bioinformatics and process systems engineering. Manuscripts concerning the design, simulation, experimental validation, control, and economic as well as ecological evaluation of novel processes using biosystems or parts thereof (e.g., enzymes, microorganisms, mammalian cells, plant cells, or tissue), their related products, or technical devices are also encouraged. The Editors will consider papers for publication based on novelty, their impact on biotechnological production and their contribution to the advancement of bioprocess and biosystems engineering science. Submission of papers dealing with routine aspects of bioprocess engineering (e.g., routine application of established methodologies, and description of established equipment) are discouraged.