Biocontrol activities and mechanisms of endophytic Bacillus licheniformis SY41 against Atractylodes chinensis root rot

IF 3.9 2区农林科学 Q1 HORTICULTURE

Scientia Horticulturae Pub Date : 2025-02-01 DOI:10.1016/j.scienta.2025.113995

Siyuan Xie , He Si , Shiqiang Wang , Yizhong Duan , Zhezhi Wang , Junfeng Niu

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引用次数: 0

Abstract

Atractylodes chinensis is a important perennial medicinal plant in East Asia. Root rot, a devastating disease, severely reduces its yield and quality. Plant endophytes may be an effective biological control agent without detrimental effects on the environment or non-target organisms. In this study, 32 endophytic bacterial strains were isolated from healthy A. chinensis root tissues. Among them, strain SY41 exhibited the most potent inhibitory effects against F. oxysporum, F. solani, and F. redolens. Strain SY41 was identified as Bacillus licheniformis, demonstrating broad-spectrum antimicrobial activity against various phytopathogenic fungi. Additionally, strain SY41 significantly reduced the germination of pathogenic spores. Transmission electron microscopy revealed that cell-free supernatants (CFS) of strain SY41 severely damaged the cell wall structures of mycelium. VOCs produced by strain SY41 disrupted the integrity of the cell membrane of pathogenic fungi, leading to the leakage of intracellular contents. Meanwhile, they induced elevated intracellular MDA levels and the accumulation of ROS in pathogenic fungi. Transcriptome analysis indicated that the genes related to cell wall and cell membrane formation, ABC (ATP-binding cassette) transporter proteins, and virulence were significantly downregulated in F. oxysporum following VOC treatment. In summary, strain SY41 inhibited pathogenic fungi through various mechanisms of action, making it a potential candidate for controlling A. chinensis root rot.

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来源期刊

Scientia Horticulturae 农林科学-园艺

CiteScore

8.60

自引率

4.70%

发文量

796

审稿时长

47 days

期刊介绍： Scientia Horticulturae is an international journal publishing research related to horticultural crops. Articles in the journal deal with open or protected production of vegetables, fruits, edible fungi and ornamentals under temperate, subtropical and tropical conditions. Papers in related areas (biochemistry, micropropagation, soil science, plant breeding, plant physiology, phytopathology, etc.) are considered, if they contain information of direct significance to horticulture. Papers on the technical aspects of horticulture (engineering, crop processing, storage, transport etc.) are accepted for publication only if they relate directly to the living product. In the case of plantation crops, those yielding a product that may be used fresh (e.g. tropical vegetables, citrus, bananas, and other fruits) will be considered, while those papers describing the processing of the product (e.g. rubber, tobacco, and quinine) will not. The scope of the journal includes all horticultural crops but does not include speciality crops such as, medicinal crops or forestry crops, such as bamboo. Basic molecular studies without any direct application in horticulture will not be considered for this journal.