Dietary DHA Regulated the Androgen Production in Male Chinese Tongue Sole Cynoglossus semilaevis

IF 3 2区 农林科学 Q1 FISHERIES
Jiahao Liu, Qiang Ma, Feiran Zhang, Qingyan Gao, Zhijun Zhang, Yuliang Wei, Mengqing Liang, Houguo Xu
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Abstract

Long-chain polyunsaturated fatty acids (LC-PUFA) play key roles in sex steroid hormone synthesis in fish. Regarding docosahexaenoic acid (DHA), currently the regulating effects were mostly evaluated in female fish rather than males. This study aimed to investigate the DHA effects on the sex steroid hormone production in male Chinese tongue sole. Three experimental diets were prepared, containing different DHA levels: a control (C) group (5.16% DHA of total fatty acids [TFA]), a low DHA group (DHA-L, 8.93%), and a high DHA group (DHA-H, 16.47%). A 58-day feeding experiment was conducted, and each diet was fed to triplicate tanks of fish. Additionally, an in vitro study with Leydig’s cells of this species was conducted to validate the in vivo results. The concentration of testosterone (T) and 11-ketotestosterone (11-KT) in the serum increased with increasing levels of dietary DHA. Dietary DHA significantly upregulated the expression of steroid hormone biosynthetic genes p450c17, hsd17b1, hsd3b1, aromatase, hsd11b2, and p45011b in the testis. The protein expression of Hsd17b1 in the testis of the DHA-H group was significantly higher compared to the other two groups, while the expression level of P450c17 showed an increasing trend with increasing dietary DHA levels. However, the in vitro results confirmed that the final concentration of DHA at 50 μmol/L could significantly increase the gene expression of p450c17 in Leydig’s cells. In conclusion, dietary DHA may promote the synthesis of T and 11-KT through the regulation of protein (Hsd17b1 and P450c17) and gene (p450c17, hsd17b1, hsd3b1, aromatase, hsd11b2, and p45011b) expression of a series of key steroid hormone biosynthetic enzymes in male Chinese tongue sole.

Abstract Image

膳食DHA对半舌鳎雄性雄性激素分泌的调节作用
长链多不饱和脂肪酸(LC-PUFA)在鱼类性类固醇激素的合成中起着关键作用。关于二十二碳六烯酸(DHA)的调节作用,目前主要是对雌性鱼的调节作用而不是对雄性鱼的调节作用。本研究旨在探讨DHA对中国舌鳎雄性类固醇激素分泌的影响。配制3种不同DHA水平的试验饲粮:对照组(C)组(DHA占总脂肪酸[TFA]的5.16%)、低DHA组(DHA- l, 8.93%)和高DHA组(DHA- h, 16.47%)。进行为期58 d的饲养试验,每种饲料分别投喂3个重复鱼缸。此外,对该物种的Leydig细胞进行了体外研究,以验证体内结果。血清睾酮(T)和11-酮睾酮(11-KT)浓度随饲料DHA水平的升高而升高。饲粮DHA显著上调睾丸激素生物合成基因p450c17、hsd17b1、hsd3b1、芳香化酶、hsd11b2和p45011b的表达。DHA- h组睾丸中Hsd17b1蛋白表达量显著高于其他两组,而P450c17蛋白表达量随饲料DHA水平的升高呈上升趋势。而体外实验结果证实,最终浓度为50 μmol/L的DHA可显著提高Leydig细胞中p450c17基因的表达。综上所述,饲料中DHA可能通过调节雄性中国舌鳎一系列类固醇激素关键生物合成酶的蛋白(Hsd17b1和P450c17)和基因(P450c17、Hsd17b1、hsd3b1、芳香化酶、hsd11b2和p45011b)表达促进T和11-KT的合成。
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来源期刊
Aquaculture Nutrition
Aquaculture Nutrition 农林科学-渔业
CiteScore
7.20
自引率
8.60%
发文量
131
审稿时长
3 months
期刊介绍: Aquaculture Nutrition is published on a bimonthly basis, providing a global perspective on the nutrition of all cultivated aquatic animals. Topics range from extensive aquaculture to laboratory studies of nutritional biochemistry and physiology. The Journal specifically seeks to improve our understanding of the nutrition of aquacultured species through the provision of an international forum for the presentation of reviews and original research papers. Aquaculture Nutrition publishes papers which strive to: increase basic knowledge of the nutrition of aquacultured species and elevate the standards of published aquaculture nutrition research. improve understanding of the relationships between nutrition and the environmental impact of aquaculture. increase understanding of the relationships between nutrition and processing, product quality, and the consumer. help aquaculturalists improve their management and understanding of the complex discipline of nutrition. help the aquaculture feed industry by providing a focus for relevant information, techniques, tools and concepts.
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