RS4 Type Resistant Starch Improves Type 2 Diabetes Mellitus in Mice by Interacting With Lactobacillus johnsonii

IF 6.9 Q1 FOOD SCIENCE & TECHNOLOGY

Food frontiers Pub Date : 2024-12-16 DOI:10.1002/fft2.528

Tao Xu, Yongheng Zhong, Shengyang Ji, Hang Xiao, Jun Li, Kaimian Li, Peiwu Li, Baiyi Lu

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Abstract

RS4-type resistant starches have been applied in food industry as thickeners or studied as functional foods for a long time. However, the influence and mechanism of RS4 on Type 2 diabetes mellitus (T2DM) have not been elucidated. In this study, sinapic acid starch ester (SASE) was chosen as a representative of functional RS4, and its anti-diabetic activity and underlying mechanism were investigated. Results showed that SASE administration lowered blood glucose and ameliorated insulin resistance of streptozotocin-induced diabetic mice via increasing serum butyrate level. Fecal microbiota transplantation confirmed the T2DM-improving activity of SASE modulated gut microbiota. 16S rRNA gene sequencing indicated that SASE selectively promoted the proliferation of butyrate-producing bacterium. In vitro co-culture of promoted gut bacterium with SASE revealed that Lactobacillus johnsonii was one of the key bacteria in the degradation of SASE. In conclusion, RS4 could selectively promote gut bacteria and increase short-chain fatty acid production to alleviate T2DM.

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RS4型抗性淀粉与约氏乳杆菌相互作用改善小鼠2型糖尿病

rs4型抗性淀粉作为增稠剂应用于食品工业或作为功能性食品研究已久。然而，RS4对2型糖尿病（T2DM）的影响及其机制尚不清楚。本研究选择sinapic acid starch ester （SASE）作为功能性RS4的代表，探讨其抗糖尿病活性及其机制。结果表明，SASE可通过提高血清丁酸盐水平降低链脲佐菌素诱导的糖尿病小鼠的血糖，改善胰岛素抵抗。粪便微生物群移植证实了SASE调节的肠道微生物群改善t2dm的活性。16S rRNA基因测序结果表明，SASE选择性促进丁酸产菌的增殖。促生菌与SASE体外共培养结果表明，约氏乳杆菌是SASE降解的关键菌之一。综上所述，RS4可选择性促进肠道菌群，增加短链脂肪酸的产生，从而缓解T2DM。

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