Denise Dozio, Sharmila Ghosh, Daniele Alessandro Consolini, Josè Luis Ermini Starna, Loris Pinto, Federico Baruzzi, Martina Letizia Contente, Andrea Pinto, Paolo Cortesi, Salvatore Princiotto, Andrea Kunova, Sabrina Dallavalle
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引用次数: 0
Abstract
Background
Plants have developed multiple chemical defence responses against pathogen attacks. The main mechanism of defence is based on a rapid transcriptional reprogramming of genes encoding biosynthetic enzymes that synthesize specific secondary metabolites. Increasing evidence indicates phenylamides (PAs) as an important group of bioactive compounds in food plants.
Results
We synthesized a small collection of ferulic acid-derived phenylamides by chemoenzymatic approaches. The compounds were tested against fungal and bacterial pathogens to assess their antimicrobial potential. The treatment with the synthesized phenylamides showed modest inhibition of the fungal growth (up to 25%) and had no significant influence on spore germination, whereas some of the compounds gave a considerable inhibition of Pyricularia oryzae appressorium formation, up to 94%. They also exhibited in vitro antibacterial activity against six foodborne bacterial pathogens. Monitoring of six growth parameters (taking into account growth rate, time and absorbance) measured during 24 h incubation showed that the synthesized molecules, assayed at four concentrations between 12.5 and 100 mg/L, produced a stronger average antimicrobial effect against Gram-positive pathogenic strains than against Gram-negative ones.
Conclusions
The obtained results evidenced that the effect of this class of compounds is mainly related to blocking fungal virulence mechanisms, mediated by a significant effect on appressorium maturation, rather than to mycelium growth inhibition. Together with the observed in vitro antibacterial activity against foodborne bacterial pathogens, we conclude that PAs are promising candidates for future developments in the agri-food sector.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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