Lingzhong Wan, Hui Sun, Ying Ni, Jun Tang, Junjie Yu, Juan Wu, Xiaoming Yan, Xiaonan Deng
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引用次数: 0
Abstract
Background
The analysis of changes in subcellular water distribution during mushroom fruiting is essential for elucidating the movement of water molecules within subcellular compartments. However, prior research on mushrooms has predominantly concentrated on alterations in water status during drying and postharvest processes in the food processing sector. Knowledge regarding subcellular water compartments throughout mushroom growth and fruiting remains limited. In the present study, the dynamics of subcellular water status across various growth stages of Agaricus bisporus were investigated using LF-NMR relaxometry.
Results
Three components were resolved from transverse relaxation curves, assigned to cell wall, cytoplasmic and vacuolar water, in both whole mushroom and mushroom tissues (stalk and Pileus). As fruiting body developed, the proton degree of freedom of three water fractions determined by T2 measurement all increased. The T2 values of three water fractions in stalk were higher than those in pileus during the first three stages, whereas they became lower compared to those in pileus from somewhere between the two stages of 2–3 and 3–4. Apparently different patterns of change in three water contents were observed, indicating the variations in water distribution at subcellular level. Furthermore, relative humidities caused obvious changes in water status. In addition, highly significant correlations were observed between T2 and textural parameters, indicating that the dynamics of water status exert a substantial influence on the formation of mushroom quality.
Conclusions
A consistent increase in the transverse proton degree of freedom of three distinct water fractions, accompanied by markedly divergent patterns in the variations of the three water contents, was observed across different growth stages of fruiting bodies. Subsequently, highly significant correlations between T2 and textural parameters were established. This study would contribute to reveal macroscopic water transport within mushroom tissues and provide theoretical insights for optimizing high-quality mushroom cultivation.
期刊介绍：
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.
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