Automatically Signal-Enhanced Lateral Flow Immunoassay for Ultrasensitive Salivary Cortisol Detection

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-02-02 DOI:10.1021/acs.analchem.4c04700

Seonjong Kim, Min-Gon Kim

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引用次数: 0

Abstract

Competitive lateral flow immunoassay (LFI) is a commonly used platform for detecting cortisol and other small molecules. However, its low sensitivity is a critical limitation for application in point-of-care tests. Thus, various signal enhancement methods have been developed, requiring complex operational steps and specialized detectors. In this study, we propose an automatic signal-enhanced LFI (asLFI), providing 100 times improved colorimetric sensitivity by applying 10,000-fold fewer AuNP conjugates compared to conventional competitive LFI, involving a single operational step. The incorporation of fewer AuNP conjugates improves the sensitivity of competitive LFI but does not produce a visible signal spontaneously. asLFI facilitates automatic signal enhancement without requiring additional operational steps, affording visible colorimetric signals. asLFI demonstrated high sensitivity in detecting cortisol (limit of detection = 3.8 pg mL^–1) across a detection range of 0.01–10 ng mL^–1 with linearity correlation (R² = 0.9159). Furthermore, a strong correlation with ELISA was observed, validating the performance of the asLFI sensor with 18 human saliva samples (R² = 0.9763). This novel asLFI sensor offers simple operation, affording ultrasensitive point-of-care tests for the circadian monitoring of cortisol.

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自动信号增强侧流免疫分析法用于超灵敏唾液皮质醇检测

竞争侧流免疫分析法（LFI）是一种常用的检测皮质醇和其他小分子的平台。然而，它的低灵敏度是在护理点测试应用的一个关键限制。因此，各种信号增强方法已经开发出来，需要复杂的操作步骤和专门的探测器。在这项研究中，我们提出了一种自动信号增强LFI (asLFI)，与传统的竞争性LFI相比，通过使用10,000倍的AuNP共轭物，只涉及一个操作步骤，就能提供100倍的比色灵敏度。较少的AuNP共轭物的掺入提高了竞争LFI的灵敏度，但不能自发地产生可见信号。asLFI有助于自动信号增强，无需额外的操作步骤，提供可见的比色信号。在0.01 ~ 10 ng mL-1的检测范围内，asLFI对皮质醇的检测灵敏度高（检测限为3.8 pg mL-1），线性相关（R2 = 0.9159）。结果表明，该传感器与酶联免疫吸附测定（ELISA）具有较强的相关性（R2 = 0.9763）。这种新颖的asLFI传感器提供简单的操作，为皮质醇的昼夜监测提供超灵敏的护理点测试。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.