Ecdysteroids in Native Trees and Callus Cultures of the Genus Vitex L. of the Flora of Russia and Vietnam.

IF 3 3区生物学 Q2 BIOCHEMICAL RESEARCH METHODS

Phytochemical Analysis Pub Date : 2025-06-01 Epub Date: 2025-01-29 DOI:10.1002/pca.3511

V V Volodin, S O Volodina, E V Nekrasova, Vu Thi Loan, E B Serebryakov, D A Frolova, K G Ufimtsev

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Abstract

Introduction: Vitex L. is a large genus of tropical and subtropical trees used in medicine of many nations. Some species are used in gynecology due to flavonoids, iridoids, and diterpenes. Other species were shown to contain ecdysteroids and are used as tonic remedies.

Objective: This study aimed to investigate content and patterns of distribution of ecdysteroids in native trees and their accumulation in in vitro cell cultures as potential biotechnological agents of Vitex species growing in remote flora of Russia and Vietnam.

Methodology: Targeted UPLC-MS/MS was used for the quantitation of the ecdysteroids in samples of plants and callus cultures of Vitex species. Determination of nucleotide sequences was carried out on ABI PRISM 3730xl sequencer. Phylogenetic tree was constructed using Mega v.7.0.

Results: Samples of Vitex agnus-castus L. from Russian Black Sea coast of the Caucasus, as well as samples of nine species of Vitex from Vietnam were collected for ecdysteroids quantification. Relationships between the content of ecdysteroids and the systematic position of the Vitex species were shown.

Conclusions: Higher levels of ecdysteroids were revealed in different plant parts of Vitex canescens Kurz, Vitex pinnata L., Vitex quinata (Lour.) F.N.Williams, and Vitex tripinnata (Lour.) Merr. It has been established that species rich in ecdysteroids, as well as those containing trace amount of ecdysteroids (V. agnus-castus L., Vitex negundo L., and Vitex rotundifolia L.f.) are grouped in certain clades isolated from each other on the molecular phylogenetic tree of the genus. The most promising raw materials are the young leaves of V. tripinnata (8562 μg/g of dry biomass), as well as the bark of V. canescens (16,520 μg/g) and V. quinata (19,130 μg/g). The bark of V. canescens could be a good source of turkesterone (11α-oxyecdysteroid) that has pronounced pharmacological effects. Its content reaches up to 1400 μg/g in the bark. Discovering ability of callus cultures for synthesis of ecdysteroids allows us to outline experimental ways to increase the level of ecdysteroid biosynthesis in biotechnological systems as alternative source of ecdysteroids.

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俄罗斯和越南植物区系牡荆属原生树木和愈伤组织培养中的表皮甾体。

简介：牡荆属是热带和亚热带树木的一个大属，在许多国家都有药用价值。由于类黄酮、环烯醚萜和二萜，一些品种被用于妇科。其他物种被证明含有表皮甾体，并被用作滋补药物。目的：研究俄罗斯和越南偏远地区牡荆属植物表皮甾体激素的含量、分布规律及其体外细胞培养积累情况。方法：采用靶向超高效液相色谱-质谱联用技术对牡荆属植物和愈伤组织培养物中表皮甾体激素进行定量分析。核苷酸序列测定采用ABI PRISM 3730xl测序仪。使用Mega v.7.0构建系统发育树。结果：采集了俄罗斯高加索黑海沿岸的牡荆花和越南的9种牡荆花的外皮甾体含量。揭示了甾体激素含量与牡荆属植物系统位置的关系。结论：白荆、山楂、藜荆不同部位表皮甾体激素含量较高。F.N.Williams和三羽牡荆（Lour.）稳定。在该属的分子系统发育树上，富含外皮甾体和含有微量外皮甾体的物种(V. agnus-castus L.、牡荆花（veex negundo L.）和圆叶牡荆花（veex rotundifolia L.f.）被归为相互分离的分支。最有潜力的原料是三羽甘薯（V. tripinnata）的幼叶（干生物量8562 μg/g），以及V. canescens的树皮（16520 μg/g）和V. quinata的树皮（19130 μg/g）。蛇皮可能是土耳其酮（11α-氧皮质激素）的良好来源，具有明显的药理作用。其含量可达1400 μg/g。发现愈伤组织培养物合成外皮甾体的能力使我们能够概述实验方法，以提高生物技术系统中作为外皮甾体替代来源的外皮甾体的生物合成水平。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Phytochemical Analysis 生物-分析化学

CiteScore

6.00

自引率

6.10%

发文量

审稿时长

1.7 months

期刊介绍： Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.