Accumulation of Water-Soluble Polysaccharides during Lychee Pulp Fermentation with Lactiplantibacillus plantarum Involves Endoglucanase Expression

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-01-27 DOI:10.1021/acs.jafc.4c08859

Guitao Huang, Dongxiao Su, Yuan-kun Lee, Xiaoqin Zou, Lihong Dong, Mei Deng, Ruifen Zhang, Fei Huang, Mingwei Zhang

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Abstract

In the current work, lychee pulp was subjected to Lactiplantibacillus plantarum ATCC 14917 fermentation, leading to a substantial increase (2.32–2.67-fold) in water-soluble polysaccharides (WSP). Concurrently, a significant degradation occurred in water-insoluble polysaccharides (WISP) composed of glucose (28.73%), arabinose (28.25%), galacturonic acid (25.07%), and galactose (11.00%). To clarify polysaccharide conversion and its relevant mechanism, carbohydrate-active enzyme encoding sequences in the L. plantarum ATCC 14917 genome and structural alterations of two polysaccharide fractions were dissected. By integrating the transcriptional assay, prokaryotic expression, and enzymic hydrolysis, three endoglucanases were demonstrated to catalyze WISP degradation, leading to WSP accumulation during lychee pulp fermentation with L. plantarum ATCC 14917. Reductions in proportions of galactose and galacturonic acid in WSPs were partly attributed to the actions of multiple galactosidases. These findings provide an enzyme-based explanation for WSP accumulation during Lactobacillus fermentation and serve as a practical foundation for directional polysaccharide conversion.

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.