Qualitative and Comparative Study of APORT Apple Peel Based on UPLC-Q-TOF-MS Technology and Fruit Peel Comparison of Different Provenances.

IF 3 3区 生物学 Q2 BIOCHEMICAL RESEARCH METHODS
Xinyue Dong, Ge Bai, Alzhan Amantay, Xiaoyu Zhang, Haiqi Zhao, Xiaofeng Ma, Zhongbo Liu
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引用次数: 0

Abstract

Objective: This study aimed to qualitatively study the main chemical components of apple peel in APORT, Kazakhstan, by ultra-performance liquid chromatography-quadrupole-time-of-flight mass spectrometry (UPLC-Q-TOF-MS/MS) and to compare the components of apple peels with different provenances.

Methods: An ACQUITY UPLC HSS T3 (100 mm × 2.1 mm, 1.8 μm) column was used with 0.1% formic acid water (A)-acetonitrile (B) as the mobile phase and gradient elution at a flow rate of 0.2 mL/min and a column temperature of 40°C. Mass spectrometry analysis was performed using the ESI positive and negative ion modes to acquire the data.

Results: With the Mass Lynx 4.2 workstation, online database, retention time of chromatographic peaks of each compound, and secondary fragmentation law, 14 compounds were identified from APORT apple peel extraction samples for the first time, and the main component categories included flavonols, organic acids, dihydrochalcones, terpenes, flavanols, etc., and caffeic acid, crataegolic acid, quinine acid, and quercitrin were found to be unique in APORT apple peel.

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来源期刊
Phytochemical Analysis
Phytochemical Analysis 生物-分析化学
CiteScore
6.00
自引率
6.10%
发文量
88
审稿时长
1.7 months
期刊介绍: Phytochemical Analysis is devoted to the publication of original articles concerning the development, improvement, validation and/or extension of application of analytical methodology in the plant sciences. The spectrum of coverage is broad, encompassing methods and techniques relevant to the detection (including bio-screening), extraction, separation, purification, identification and quantification of compounds in plant biochemistry, plant cellular and molecular biology, plant biotechnology, the food sciences, agriculture and horticulture. The Journal publishes papers describing significant novelty in the analysis of whole plants (including algae), plant cells, tissues and organs, plant-derived extracts and plant products (including those which have been partially or completely refined for use in the food, agrochemical, pharmaceutical and related industries). All forms of physical, chemical, biochemical, spectroscopic, radiometric, electrometric, chromatographic, metabolomic and chemometric investigations of plant products (monomeric species as well as polymeric molecules such as nucleic acids, proteins, lipids and carbohydrates) are included within the remit of the Journal. Papers dealing with novel methods relating to areas such as data handling/ data mining in plant sciences will also be welcomed.
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