Use of digital image analysis to improve rigor and efficiency of physeal bone growth measurements.

IF 2 4区 生物学 Q3 CELL BIOLOGY
Histology and histopathology Pub Date : 2025-09-01 Epub Date: 2025-01-17 DOI:10.14670/HH-18-875
Grant Ozaki, Jay Byrd, Bria Foley, Alexander Farell, Garret Williams, Max Twedt, James Sypherd, Ellen Leiferman, Cameron Jeffers, Matthew A Halanski
{"title":"Use of digital image analysis to improve rigor and efficiency of physeal bone growth measurements.","authors":"Grant Ozaki, Jay Byrd, Bria Foley, Alexander Farell, Garret Williams, Max Twedt, James Sypherd, Ellen Leiferman, Cameron Jeffers, Matthew A Halanski","doi":"10.14670/HH-18-875","DOIUrl":null,"url":null,"abstract":"<p><p>In larger, translational animal models, manual measurements of longitudinal bone growth using fluorochrome labels is tedious and may be prone to less rigor due to variations in reader experience, sampling differences, and photobleaching that limits the repeatability of measurements. This study assesses the reliability of three different digital methods to assist in measurement of distance between pulsed fluorochrome labels. Forty-five tibial physes from skeletally immature New Zealand White rabbits were pulsed with fluorochrome labels and measured using Fully Manual Technique (FMT), Manual Digital Measurement (MDM), Computer Assisted Image Processing (AIP), and Fully Automated Measurement (FAM). Pearson's correlation coefficient and Bland-Altman analysis were used to analyze the different methods. Intraclass correlation coefficient (ICC) assessed inter- and intra-reader reliability. Regardless of the method, all growth rate measurement techniques exhibited excellent agreement and reliability. The computer assisted methods allowed rapid data acquisition without compromising reliability, thereby improving efficiency in bone growth research. Clinical Significance: Distances between fluorochrome bone labels on large samples can be reliably measured using digital imaging and processing techniques.</p>","PeriodicalId":13164,"journal":{"name":"Histology and histopathology","volume":" ","pages":"1397-1404"},"PeriodicalIF":2.0000,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Histology and histopathology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.14670/HH-18-875","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/1/17 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
引用次数: 0

Abstract

In larger, translational animal models, manual measurements of longitudinal bone growth using fluorochrome labels is tedious and may be prone to less rigor due to variations in reader experience, sampling differences, and photobleaching that limits the repeatability of measurements. This study assesses the reliability of three different digital methods to assist in measurement of distance between pulsed fluorochrome labels. Forty-five tibial physes from skeletally immature New Zealand White rabbits were pulsed with fluorochrome labels and measured using Fully Manual Technique (FMT), Manual Digital Measurement (MDM), Computer Assisted Image Processing (AIP), and Fully Automated Measurement (FAM). Pearson's correlation coefficient and Bland-Altman analysis were used to analyze the different methods. Intraclass correlation coefficient (ICC) assessed inter- and intra-reader reliability. Regardless of the method, all growth rate measurement techniques exhibited excellent agreement and reliability. The computer assisted methods allowed rapid data acquisition without compromising reliability, thereby improving efficiency in bone growth research. Clinical Significance: Distances between fluorochrome bone labels on large samples can be reliably measured using digital imaging and processing techniques.

使用数字图像分析提高骨骺生长测量的严谨性和效率。
在较大的平移动物模型中,使用荧光标记进行纵向骨生长的手动测量是繁琐的,并且由于读者经验的变化,采样差异和限制测量可重复性的光漂白,可能容易不那么严格。本研究评估了三种不同的数字方法的可靠性,以协助测量脉冲荧光标签之间的距离。对45只未成熟新西兰大白兔的胫骨进行荧光标记脉冲,并采用全手工技术(FMT)、手工数字测量(MDM)、计算机辅助图像处理(AIP)和全自动测量(FAM)进行测量。采用Pearson相关系数分析法和Bland Altman分析法对不同方法进行分析。类内相关系数(ICC)评估阅读器间和阅读器内的信度。无论采用何种方法,所有生长速率测量技术均表现出良好的一致性和可靠性。计算机辅助方法允许快速数据采集而不影响可靠性,从而提高骨生长研究的效率。临床意义:使用数字成像和处理技术可以可靠地测量大样本上荧光骨标记之间的距离。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Histology and histopathology
Histology and histopathology 生物-病理学
CiteScore
3.90
自引率
0.00%
发文量
232
审稿时长
2 months
期刊介绍: HISTOLOGY AND HISTOPATHOLOGY is a peer-reviewed international journal, the purpose of which is to publish original and review articles in all fields of the microscopical morphology, cell biology and tissue engineering; high quality is the overall consideration. Its format is the standard international size of 21 x 27.7 cm. One volume is published every year (more than 1,300 pages, approximately 90 original works and 40 reviews). Each volume consists of 12 numbers published monthly online. The printed version of the journal includes 4 books every year; each of them compiles 3 numbers previously published online.
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:604180095
Book学术官方微信