Molecular and Immunological Characterization of Troponin C: An Allergen from Scylla paramamosain

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-01-23 DOI:10.1021/acs.jafc.4c10877

Fei Xia, Mengsi Li, Qingmei Liu, Yuni Liao, Fajie Li, Xinyu Han, Chih-Jung Chang, Lianzhong Luo, Yixiang Liu, Guangming Liu

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Abstract

Scylla paramamosain, a crustacean of substantial importance, is a frequent trigger of food allergies. This study examined the molecular and immunological properties of troponin C from S. paramamosain (Scy p TnC) as an allergen. The findings indicated that thermal stability of Scy p TnC comprised 150 amino acids and facilitated the induction of CD63/CD203c in basophils from crab allergy patients. Furthermore, treatment of Scy p TnC with chemical denaturants caused structural degradation, which resulted in diminished IgG binding capacity. Subsequently, 6 linear epitopes and 4 conformational epitope regions of TnC were predicted, with epitopes at the C-terminal being conserved throughout 9 discovered TnCs. Concurrently, mice sensitized with Scy p TnC exhibited markedly increased levels of IgE and IL-4 release, provoking a Th2 immune response. The results reveal crab allergens and enhance existing knowledge regarding allergenic components in crabs, thereby facilitating the advancement of molecular diagnostics and targeted therapeutic strategies.

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来自Scylla paramamosain的过敏原肌钙蛋白C的分子和免疫学特性

Scylla paramosain是一种非常重要的甲壳类动物，它经常引发食物过敏。本研究研究了s.p amosain （Scy p TnC）作为过敏原的肌钙蛋白C的分子和免疫学特性。结果表明，scyp TnC的热稳定性包括150个氨基酸，促进了螃蟹过敏患者嗜碱性细胞CD63/CD203c的诱导。此外，用化学变性剂处理scyp TnC会导致结构降解，导致IgG结合能力降低。随后，预测了TnC的6个线性表位和4个构象表位区域，在9个发现的TnC中，c端表位是保守的。同时，scyp TnC致敏小鼠表现出显著增加的IgE和IL-4释放水平，引发Th2免疫反应。研究结果揭示了螃蟹过敏原，增强了对螃蟹过敏原成分的现有认识，从而促进了分子诊断和靶向治疗策略的发展。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.