Polyfluorene-Enhanced Near-Infrared Electrochemiluminescence of Heptamethine Cyanine Dye for Coreactants-Free Bioanalysis

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-01-22 DOI:10.1021/acs.analchem.4c04722

Xin Wen, Yingying Chen, Ying He, Ruo Yuan, Shihong Chen

{"title":"Polyfluorene-Enhanced Near-Infrared Electrochemiluminescence of Heptamethine Cyanine Dye for Coreactants-Free Bioanalysis","authors":"Xin Wen, Yingying Chen, Ying He, Ruo Yuan, Shihong Chen","doi":"10.1021/acs.analchem.4c04722","DOIUrl":null,"url":null,"abstract":"The near-infrared electrochemiluminescence (NIR-ECL) technique has received special attention in cell imaging and biomedical analysis due to its deep tissue penetration, low background interference, and high sensitivity. Although cyanine-based dyes are promising NIR-ECL luminophores, limited ECL efficiency and the need for exogenous coreactants have prevented their widespread application. In this work, poly[9,9-bis(3′-(<i>N,N</i>-dimethylamino)propyl)-2,7-fluorene]-<i>alt</i>-2,7-(9,9-dioctylfluorene)] (PFN) was innovatively developed to significantly invigorate the NIR-ECL performance of heptamethine cyanine dye IR 783 by the resonance energy transfer (RET) strategy. Astonishingly, the IR@PFN nanoparticles (NPs) synthesized from IR 783 and PFN by a nanoprecipitation method emitted a strong coreactant-free NIR-ECL signal at +1.05 V, and the maximum emission wavelength was 815 nm. IR@PFN NPs were integrated in a spontaneous entropy-driven chain replacement (ESDR) reaction to achieve ECL analysis of microRNA-21 (miRNA-21), and the limit of detection was as low as 0.25 fM. IR@PFN NPs created a promising coreactant-free NIR-ECL platform for bioanalysis and imaging, providing a novel NIR-ECL detection method for miRNA-21.","PeriodicalId":27,"journal":{"name":"Analytical Chemistry","volume":"19 1","pages":""},"PeriodicalIF":6.7000,"publicationDate":"2025-01-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Chemistry","FirstCategoryId":"92","ListUrlMain":"https://doi.org/10.1021/acs.analchem.4c04722","RegionNum":1,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}

引用次数: 0

Abstract

The near-infrared electrochemiluminescence (NIR-ECL) technique has received special attention in cell imaging and biomedical analysis due to its deep tissue penetration, low background interference, and high sensitivity. Although cyanine-based dyes are promising NIR-ECL luminophores, limited ECL efficiency and the need for exogenous coreactants have prevented their widespread application. In this work, poly[9,9-bis(3′-(N,N-dimethylamino)propyl)-2,7-fluorene]-alt-2,7-(9,9-dioctylfluorene)] (PFN) was innovatively developed to significantly invigorate the NIR-ECL performance of heptamethine cyanine dye IR 783 by the resonance energy transfer (RET) strategy. Astonishingly, the IR@PFN nanoparticles (NPs) synthesized from IR 783 and PFN by a nanoprecipitation method emitted a strong coreactant-free NIR-ECL signal at +1.05 V, and the maximum emission wavelength was 815 nm. IR@PFN NPs were integrated in a spontaneous entropy-driven chain replacement (ESDR) reaction to achieve ECL analysis of microRNA-21 (miRNA-21), and the limit of detection was as low as 0.25 fM. IR@PFN NPs created a promising coreactant-free NIR-ECL platform for bioanalysis and imaging, providing a novel NIR-ECL detection method for miRNA-21.

Abstract Image

查看原文本刊更多论文

聚芴增强近红外电化学发光用于无反应物生物分析的七甲基菁染料

近红外电化学发光（NIR-ECL）技术因其组织穿透深度大、背景干扰小、灵敏度高等优点，在细胞成像和生物医学分析领域受到了广泛的关注。尽管基于花青素的染料是很有前途的NIR-ECL发光团，但有限的ECL效率和对外源共物的需求阻碍了它们的广泛应用。本研究通过共振能量转移（RET）策略，创新制备了聚[9,9-二（3′-（N，N-二甲氨基）丙基）-2,7-（9,9-二辛基芴）](PFN)，以显著提高七甲基菁染料IR 783的NIR-ECL性能。令人惊讶的是，由IR 783和PFN通过纳米沉淀法合成的IR@PFN纳米粒子（NPs）在+1.05 V下发射出强的无反应物NIR-ECL信号，最大发射波长为815 nm。IR@PFN NPs整合到自发熵驱动链替换（ESDR）反应中，实现对microRNA-21 （miRNA-21）的ECL分析，检出限低至0.25 fM。IR@PFN NPs为生物分析和成像创造了一个有前途的无反应物NIR-ECL平台，为miRNA-21提供了一种新的NIR-ECL检测方法。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

求助全文

约1分钟内获得全文求助全文

来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.