Highly Sensitive Digital and Analog Immunoassay Based on Submicron Magnetic Beads and Fluorescent Microspheres

IF 6.7 1区化学 Q1 CHEMISTRY, ANALYTICAL

Analytical Chemistry Pub Date : 2025-01-23 DOI:10.1021/acs.analchem.4c04570

Feng Gong, Xiaoyun Shan, Kebing Yi, Ziwen Tang, Yimiao He, Fuxiang Zhou, Xinghu Ji, Zhike He

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Abstract

The detection of disease-related protein biomarkers plays a crucial role in the early diagnosis, treatment, and monitoring of diseases. The concentrations of protein biomarkers can vary significantly in different diseases or stages of the same disease. However, most of the existing analytical methods cannot simultaneously meet the requirements of high sensitivity and a wide dynamic range. Herein, we developed a digital and analog immunoassay method based on submicron magnetic beads and fluorescent microspheres. The digital analysis achieves a limit of detection as low as 46 fg/mL (1.8 fM) for IL-6, and the analog analysis has a wide dynamic range spanning from 0.2 pg/mL to 10 ng/mL. Furthermore, the analog analysis can be used to quickly and roughly assess higher concentration proteins via visual detection, and the practical application potential of this method was verified by the detection of alpha-fetoprotein in serum samples from 12 healthy individuals and hepatocellular carcinoma patients. The established method does not involve the use of enzymes or costly instruments, which greatly simplifies the experimental steps, shortens the experimental time, and reduces the experimental cost. In view of those advantages, the proposed method has great application prospects in point-of-care applications.

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基于亚微米磁珠和荧光微球的高灵敏度数字和模拟免疫分析

疾病相关蛋白生物标志物的检测在疾病的早期诊断、治疗和监测中起着至关重要的作用。蛋白质生物标志物的浓度在不同疾病或同一疾病的不同阶段可能有显著差异。然而，现有的分析方法大多不能同时满足高灵敏度和宽动态范围的要求。在此，我们开发了一种基于亚微米磁珠和荧光微球的数字和模拟免疫分析方法。数字分析IL-6的检测限低至46 fg/mL (1.8 fM)，模拟分析具有0.2 pg/mL至10 ng/mL的宽动态范围。通过对12例健康人及肝癌患者血清中甲胎蛋白的检测，验证了该方法的实际应用潜力。所建立的方法不涉及使用酶或昂贵的仪器，大大简化了实验步骤，缩短了实验时间，降低了实验成本。鉴于这些优点，该方法在医疗点应用中具有很大的应用前景。

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来源期刊

Analytical Chemistry 化学-分析化学

CiteScore

12.10

自引率

12.20%

发文量

1949

审稿时长

1.4 months

期刊介绍： Analytical Chemistry, a peer-reviewed research journal, focuses on disseminating new and original knowledge across all branches of analytical chemistry. Fundamental articles may explore general principles of chemical measurement science and need not directly address existing or potential analytical methodology. They can be entirely theoretical or report experimental results. Contributions may cover various phases of analytical operations, including sampling, bioanalysis, electrochemistry, mass spectrometry, microscale and nanoscale systems, environmental analysis, separations, spectroscopy, chemical reactions and selectivity, instrumentation, imaging, surface analysis, and data processing. Papers discussing known analytical methods should present a significant, original application of the method, a notable improvement, or results on an important analyte.