Synthetic Cannabinoids are Genotoxic in Cultured Human Lymphocytes.

IF 2.6 4区医学 Q2 PHARMACOLOGY & PHARMACY

Current pharmaceutical design Pub Date : 2025-01-17 DOI:10.2174/0113816128340465241227095853

Alla Abdulwahab Almestafa, Omar Falah Khabour, Laith Naser Al-Eitan, Karem Hasan Alzoubi

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引用次数: 0

Abstract

Background: Synthetic cannabinoids are one of the most identified abused drugs nowadays. Their popularity is due to their psychoactive effects, which resemble delta 9 tetrahydrocannabinol. This study investigates the genotoxic potential of three synthetic cannabinoids of indazole-passed drugs, AB-Fubinaca, AMBFubinaca, and EMB-Fubinaca (at a final concentration of 200 nM).

Methods: Genotoxicity was examined using Sister Chromatid Exchanges (SCEs) and Chromosomal Aberrations (CAs) assays in cultured human lymphocytes. Blood for lymphocyte cultures was obtained from healthy adult young males.

Results: A significant increase in the frequency of SCEs was detected for all examined drugs (range: 5.4-6.1, p < 0.05) compared to the control group (4.70 ± 0.31). The order of synthetic cannabinoids in terms of their ability to induce SCEs was EMB-Fubinaca (6.04 ± 0.63) > AMB-Fubinaca (5.65 ± 0.6) > AB-Fubinaca (5.33 ± 0.58). None of the examined drugs induced significant changes to the frequency of CAs (p > 0.05). Additionally, there were no effects of the synthetic cannabinoids at the studied concentration on proliferation and mitotic indices.

Conclusion: Synthetic cannabinoids have been found to increase the frequency of SCEs in cultured human lymphocytes. The results should be confirmed in in vivo studies using lymphocytes derived from synthetic cannabinoid users.

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合成大麻素对培养的人淋巴细胞具有遗传毒性。

背景：合成大麻素是当今最常见的滥用药物之一。它们受欢迎的原因是它们的精神活性作用，类似于德尔塔9四氢大麻酚。本研究考察了吲哚唑通过的药物AB-Fubinaca、AMBFubinaca和EMB-Fubinaca三种合成大麻素（终浓度为200 nM）的遗传毒性潜力。方法：采用姐妹染色单体交换法（sce）和染色体畸变法（CAs）检测培养的人淋巴细胞的遗传毒性。用于淋巴细胞培养的血液取自健康的年轻成年男性。结果：与对照组（4.70±0.31）相比，各药物组SCEs检出率均显著升高（范围：5.4 ~ 6.1,p < 0.05）。合成大麻素诱导sce的能力依次为EMB-Fubinaca（6.04±0.63）、AMB-Fubinaca（5.65±0.6）、abb1 - fubinaca（5.33±0.58）。两种药物均未引起ca发生频率的显著变化（p < 0.05）。此外，在研究浓度下，合成大麻素对细胞增殖和有丝分裂指标没有影响。结论：合成大麻素可增加培养的人淋巴细胞中SCEs的发生频率。该结果应在使用合成大麻素使用者的淋巴细胞的体内研究中得到证实。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Current pharmaceutical design 医学-药学

CiteScore

6.30

自引率

0.00%

发文量

302

审稿时长

2 months

期刊介绍： Current Pharmaceutical Design publishes timely in-depth reviews and research articles from leading pharmaceutical researchers in the field, covering all aspects of current research in rational drug design. Each issue is devoted to a single major therapeutic area guest edited by an acknowledged authority in the field. Each thematic issue of Current Pharmaceutical Design covers all subject areas of major importance to modern drug design including: medicinal chemistry, pharmacology, drug targets and disease mechanism.