Evolution of interorganismal strigolactone biosynthesis in seed plants

IF 44.7 1区综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES

Science Pub Date : 2025-01-16 DOI:10.1126/science.adp0779

Anqi Zhou, Annalise Kane, Sheng Wu, Kaibiao Wang, Michell Santiago, Yui Ishiguro, Kaori Yoneyama, Malathy Palayam, Nitzan Shabek, Xiaonan Xie, David C. Nelson, Yanran Li

引用次数: 0

Abstract

Strigolactones (SLs) are methylbutenolide molecules derived from β-carotene through an intermediate carlactonoic acid (CLA). Canonical SLs act as signals to microbes and plants, whereas noncanonical SLs are primarily plant hormones. The cytochrome P450 CYP722C catalyzes a critical step, converting CLA to canonical SLs in most angiosperms. Using synthetic biology, we investigated the function of CYP722A , an evolutionary predecessor of CYP722C . CYP722A converts CLA into 16-hydroxy-CLA (16-OH-CLA), a noncanonical SL detected exclusively in the shoots of various flowering plants. 16-OH-CLA application restores control of shoot branching to SL-deficient mutants in Arabidopsis thaliana and is perceived by the SL signaling pathway. We hypothesize that biosynthesis of 16-OH-CLA by CYP722A was a metabolic stepping stone in the evolution of canonical SLs that mediate rhizospheric signaling in many flowering plants.

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种子植物间独角麦内酯生物合成的进化

己糖内酯（SLs）是一种甲基丁烯内酯分子，由β-胡萝卜素通过中间的卡拉内酯酸（CLA）衍生而来。规范SLs作为微生物和植物的信号，而非规范SLs主要是植物激素。在大多数被子植物中，细胞色素P450 CYP722C催化一个关键步骤，将CLA转化为典型的SLs。利用合成生物学的方法，我们研究了CYP722A的功能，它是CYP722C的进化前身。CYP722A将CLA转化为16-羟基-CLA (16-OH-CLA)，这是一种仅存在于各种开花植物芽中的非典型SL。16-OH-CLA的应用恢复了拟南芥SL缺陷突变体的茎分枝控制，并通过SL信号通路感知。我们假设CYP722A生物合成16-OH-CLA是许多开花植物中介导根际信号传导的典型SLs进化的代谢踏脚石。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Science 综合性期刊-综合性期刊

CiteScore

61.10

自引率

0.90%

发文量

审稿时长

2.1 months

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