Fungal Pathogen Diversity and Fungicide Resistance Assessment in Fusarium Crown Rot of Wheat in the Huanghuai Region of China

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-01-16 DOI:10.1021/acs.jafc.4c09274

Chenghui Xu, Meiling Guo, Xingmin Han, Chunjiang Ren, Chao Liu, Weitao Fu, Junshan Qi, Zhiwei Ge, Zhonghua Ma, Yun Chen

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Abstract

Fusarium crown rot (FCR) poses a major threat to wheat production in the Huanghuai wheat region of China. This study aims to enhance understanding of pathogen populations causing FCR, focusing on their pathogenicity, trichothecene genotypes, and fungicide resistance. During the 2022–2023 growing seasons, we collected 1820 fungal isolates from 233 locations in this region. Our results identified Fusarium pseudograminearum, Fusarium graminearum, and Fusarium asiaticum as the primary pathogens, with F. pseudograminearum exhibiting the highest virulence. Three trichothecene genotypes were identified, including nivalenol, 3-acetyldeoxynivalenol, and 15-acetyldeoxynivalenol. No correlation was observed between trichothecene genotype and virulence, except in F. asiaticum. Antifungal assays demonstrated that all six tested fungicides effectively inhibited F. pseudograminearum, with fludioxonil being particularly effective. Field surveys identified isolates resistant to difenoconazole and pyraclostrobin. Laboratory analysis also revealed strains with FpSdhC₁^A83 V and FpSdhC₁^S80N mutations conferring resistance to cyclobutrifluram. These findings offer critical insights for developing effective control strategies to manage FCR.

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中国黄淮地区小麦镰刀菌冠腐病的真菌病原多样性和杀菌剂抗药性评估

镰刀菌冠腐病（Fusarium crown rot， FCR）是黄淮小麦产区小麦生产的主要威胁之一。本研究旨在加强对FCR病原菌群体的了解，重点研究其致病性、菌毛菌基因型和杀菌剂抗性。在2022-2023年的生长季节，我们在该地区233个地点收集了1820株真菌分离株。结果表明，主要病原菌为伪谷物镰刀菌（Fusarium pseudograminearum）、谷物镰刀菌（Fusarium graminearum）和亚洲镰刀菌（Fusarium asiatium）。鉴定出三种毛线虫烯基因型，包括雪腐镰刀菌醇、3-乙酰脱氧雪腐镰刀菌醇和15-乙酰脱氧雪腐镰刀菌醇。除亚洲毛霉外，毛霉基因型与毒力无相关性。抗真菌试验表明，所有6种被试杀菌剂都能有效地抑制伪谷草镰刀菌，其中氟菌腈效果特别好。实地调查发现菌株对异虫康唑和嘧菌酯耐药。实验室分析还发现，携带FpSdhC1A83 V和FpSdhC1S80N突变的菌株对环丁氟仑具有耐药性。这些发现为制定有效的控制策略来管理FCR提供了重要的见解。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.