Anti-TMV Activity and Functional Mechanisms of Two Diterpenoid Alkaloids Isolated from Dendrobium findlayanum

IF 5.7 1区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of Agricultural and Food Chemistry Pub Date : 2025-01-14 DOI:10.1021/acs.jafc.4c12988

Cheng-Gang Song, Yang Yang, Wenjing Cui, Wei Chen, Shouxian Wang, Jianchun Qin, Jinliang Liu

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Abstract

Tobacco mosaic virus (TMV) is a major threat to crops, making the discovery of green biopesticides essential. Herein, we present two active ingredients derived from the medicinal plant Dendrobium findlayanum, findlayine A (1) and dendrofindline B (2), as promising precursor compounds for TMV inhibitors. Among them, 2 inhibited TMV infestation on tobacco leaves at a rate of 38.6%, which was close to that of the commercial antiviral agent ningnanmycin (43.1%). Both 1 and 2 could effectively alleviate the destruction of tobacco leaf protoplasts by TMV infestation, and enhance the resistance of tobacco to TMV by affecting the activities of phenylalaninammo-nialyase (PAL), peroxidase (POD) and superoxide dismutase (SOD). Additionally, 1 and 2 significantly down-regulated the expression of TMV coat protein (CP) and inhibited CP-mediated aggregation of TMV, thereby reducing its infestation capacity. This study systematically investigated the mechanism of anti-TMV activity of diterpenoid alkaloids from D. findlayanum, with a view to providing new insights into the creation of novel green antiviral agents.

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石斛两种二萜类生物碱抗tmv活性及作用机制研究

烟草花叶病毒（TMV）是对作物的主要威胁，因此发现绿色生物农药至关重要。本文中，我们从药用植物石斛中提取了两种活性成分，findlayine A(1)和denendrofindline B(2)，作为TMV抑制剂的前体化合物。其中2种对烟草叶片TMV侵染的抑制率为38.6%，与市售抗病毒药宁南霉素的抑制率（43.1%）接近。1和2均能通过影响苯丙氨酸氨解酶（PAL）、过氧化物酶（POD）和超氧化物歧化酶（SOD）活性，有效缓解烟草叶片原生质体受TMV侵染的破坏，增强烟草对TMV的抗性。此外，1和2显著下调TMV外壳蛋白（CP）的表达，抑制CP介导的TMV聚集，从而降低其侵染能力。本研究系统地探讨了芬德兰二萜类生物碱抗tmv的作用机制，以期为开发新型绿色抗病毒药物提供新的思路。

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来源期刊

Journal of Agricultural and Food Chemistry 农林科学-农业综合

CiteScore

9.90

自引率

8.20%

发文量

1375

审稿时长

2.3 months

期刊介绍： The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.