Correction to “Stigmasterol Activates the mTOR Signaling Pathway by Inhibiting ORP5 Ubiquitination to Promote Milk Synthesis in Bovine Mammary Epithelial Cells”

Abstract

Figure 7 as originally published was incorrect. The correct figures are given below. Funding (No. 32202766) was included incorrectly in the funding section and should be removed; the corrected Funding statement is below. The corrections do not affect the conclusions of the paper. Figure 7. ST promotes the synthesis of milk through ORP5. BMECs were transfected with ORP5-siRNA for 24 h, followed by stimulation with 10 μMST for 24 h. (A) The protein expression levels of ORP5 were detected by Western blotting. (B) The protein bands of ORP5 were quantitatively calculated with β-actin as the internal reference. (C) The content of TAG in cells was analyzed using a TAG detection kit. (D) The content of TAG in the culture medium was analyzed using a TAG detection kit.(E) Lipid droplets and nuclei were stained with BODIPY 493/503 (green) and DAPI (blue). Results were visualized using a confocal microscope (1200×, scale bar = 10 μm). (F) The area and integrated optical density (AOD) of lipid droplet spots per cell in Figure 7F were quantitatively analyzed using ImageJ software. (G, H) The cell proliferation capacity was determined using the EdU cell proliferation assay kit. (I) The protein expression levels of p-mTOR, mTOR, DGAT1, DGAT2, FASN, SREBP1, β-casein, and Cyclin D1 were detected by Western blotting. (J) The relative protein abundance of DGAT1, DGAT2, FASN, SREBP1, β-casein, and Cyclin D1 (compared to β-actin) as well as p-mTOR (compared to mTOR) from Figure 7I. All data are presented as mean ± SEM (n = 3). **p < 0.01 versus the NC group. This study was supported by the Natural Science Foundation of Jilin Province (project no. 20220101302JC). This article has not yet been cited by other publications.