Comprehensive Analysis of Fecal Microbiome and Metabolomics Uncovered dl-Norvaline-Ameliorated Obesity-Associated Disorders in High-Fat Diet-Fed Obese Mice by Targeting the Gut Microbiota

IF 5.7 1区 农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY
Xin Li, Yanting Qin, Fangfang Yue, Xin Lü
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Abstract

Norvaline is a nonproteinogenic amino acid and an important food ingredient supplement for healthy food. In this study, dl-norvaline administration reduced body weight by more than 40% and improved glucose metabolism and energy metabolism in obese mice induced by a high-fat diet (HFD). Combination analysis of microbiome and metabolomics showed that dl-norvaline supplementation regulated gut bacteria structure, such as increasing beneficial bacteria (Mollicutes_RF39, Ruminococcaceae, Bacteroidaceae, Rikenellaceae, Lactobacillaceae, Clostridiaceae_1, uncultured_bacterium_f_Muribaculaceae, and Rikenellaceae_RC9_gut_group) and decreasing harmful bacteria (Fusobacteriia, Desulfovibrionales, Enterobacteriaceae, Burkholderiaceae, Helicobacteraceae, and Veillonellaceae) and modulated the metabolites involved in arachidonic acid metabolism, thus further promoting short-chain fatty acid production and improving gut barrier, thereby inflammatory responses and oxidative stress were ameliorated. In addition, the pseudogerm-free mouse model verified that dl-norvaline ameliorated obesity-associated disorders in HFD-fed obese mice by targeting gut microbiota. These results clarified that dl-norvaline may be promising for developing and innovating potential functional food products.

Abstract Image

粪便微生物组和代谢组学综合分析发现dl-正缬氨酸可通过靶向肠道微生物组改善高脂饮食喂养肥胖小鼠的肥胖相关疾病
正缬氨酸是一种非蛋白质原性氨基酸,是健康食品的重要食品成分补充剂。在本研究中,给药dl-正缬氨酸使高脂饮食(HFD)诱导的肥胖小鼠体重减轻40%以上,并改善葡萄糖代谢和能量代谢。微生物组学和代谢组学联合分析表明,添加dl-正缬氨酸可调节肠道菌群结构,使有益菌群(Mollicutes_RF39、Ruminococcaceae、Bacteroidaceae、Rikenellaceae、Lactobacillaceae、Clostridiaceae_1、uncultured_bacterum_f_muribaculaceae、Rikenellaceae_RC9_gut_group)增加,有害菌群(Fusobacteriia、Desulfovibrionales、Enterobacteriaceae、burkholderaceae、Helicobacteraceae、),调节参与花生四烯酸代谢的代谢物,从而进一步促进短链脂肪酸的产生,改善肠道屏障,从而改善炎症反应和氧化应激。此外,假无菌小鼠模型验证了dl-正缬氨酸通过靶向肠道微生物群改善hfd喂养的肥胖小鼠的肥胖相关疾病。这些结果表明,dl-正缬氨酸在开发和创新潜在的功能食品方面具有广阔的前景。
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来源期刊
Journal of Agricultural and Food Chemistry
Journal of Agricultural and Food Chemistry 农林科学-农业综合
CiteScore
9.90
自引率
8.20%
发文量
1375
审稿时长
2.3 months
期刊介绍: The Journal of Agricultural and Food Chemistry publishes high-quality, cutting edge original research representing complete studies and research advances dealing with the chemistry and biochemistry of agriculture and food. The Journal also encourages papers with chemistry and/or biochemistry as a major component combined with biological/sensory/nutritional/toxicological evaluation related to agriculture and/or food.
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