Study on the repair function of radiation-induced salivary gland injury using human amniotic mesenchymal stem cells pre treated with hypoxia.

IF 2.2 3区医学 Q2 Dentistry

Journal of Stomatology Oral and Maxillofacial Surgery Pub Date : 2025-01-10 DOI:10.1016/j.jormas.2025.102223

Yingxin Wang, Nana Liu, Xin Zhang, Min Dai, Nini Zhang, Guilin Huang

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Abstract

Objective: To investigate the reparative effect of hypoxia pretreated hAMSCs on radiation-induced damage to salivary gland function in mice.

Methods: hAMSCs were separated from human amniotic tissues by mechanical and enzymatic digestion methods and a 15 Gy electron beam was used to locally irradiate the neck of mouse to create a salivary gland injury model. The mouse models were randomly divided into four groups: control group, IR+PBS group, IR+Nor group and IR+HP group.

Result: Two months after hAMSCs injection, the saliva flow of mice in the IR+PBS group was significantly lower than that of the control group (P < 0.05). The saliva flow of mice in the IR+Nor group and IR+HP group were significantly increased compared to the IR+PBS group (P < 0.05). The cell apoptosis rate of the IR+PBS group was sensibly higher than that of the blank control group (P < 0.05). The cell apoptosis rates of the IR+Nor group and the IR+HP group were lower than that of the IR+PBS group. In addition, the apoptosis rate of the IR+HP group was lower than that of the IR+Nor group (P < 0.05). The changes of IOD of α-Amy in each group showed that the expression of α - Amy in the IR+PBS group was significantly lower than that in the blank control group (P < 0.05). Compared with the IR+PBS group, the IR+Nor group and the IR+HP group showed an obvious increase in the expression of α-Amy (P < 0.05).

Conclusion: Low oxygen pretreatment of hAMSCs could more effectively repair the function of radiation-induced salivary gland compared to normoxic cultivation.

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缺氧预处理人羊膜间充质干细胞修复辐射性唾液腺损伤功能的研究。

目的：研究缺氧预处理的 hAMSCs 对小鼠唾液腺功能辐射损伤的修复作用：方法：采用机械和酶解方法从人羊膜组织中分离出hAMSCs，用15 Gy电子束局部照射小鼠颈部，建立唾液腺损伤模型。小鼠模型随机分为四组：对照组、IR+PBS组、IR+Nor组和IR+HP组：结果：注射 hAMSCs 两个月后，IR+PBS 组小鼠的唾液流量明显低于对照组（PC 结论：IR+PBS 组小鼠的唾液流量明显低于对照组（PC）：与常氧培养相比，低氧预处理 hAMSCs 能更有效地修复辐射引起的唾液腺功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Stomatology Oral and Maxillofacial Surgery Dentistry-Oral Surgery

CiteScore

2.20

自引率

9.10%

发文量

305

期刊介绍： J Stomatol Oral Maxillofac Surg publishes research papers and techniques - (guest) editorials, original articles, reviews, technical notes, case reports, images, letters to the editor, guidelines - dedicated to enhancing surgical expertise in all fields relevant to oral and maxillofacial surgery: from plastic and reconstructive surgery of the face, oral surgery and medicine, … to dentofacial and maxillofacial orthopedics. Original articles include clinical or laboratory investigations and clinical or equipment reports. Reviews include narrative reviews, systematic reviews and meta-analyses. All manuscripts submitted to the journal are subjected to peer review by international experts, and must: Be written in excellent English, clear and easy to understand, precise and concise; Bring new, interesting, valid information - and improve clinical care or guide future research; Be solely the work of the author(s) stated; Not have been previously published elsewhere and not be under consideration by another journal; Be in accordance with the journal''s Guide for Authors'' instructions: manuscripts that fail to comply with these rules may be returned to the authors without being reviewed. Under no circumstances does the journal guarantee publication before the editorial board makes its final decision. The journal is indexed in the main international databases and is accessible worldwide through the ScienceDirect and ClinicalKey Platforms.