Study on the repair function of radiation-induced salivary gland injury using human amniotic mesenchymal stem cells pre treated with hypoxia

IF 1.8 3区医学 Q2 DENTISTRY, ORAL SURGERY & MEDICINE

Journal of Stomatology Oral and Maxillofacial Surgery Pub Date : 2025-01-10 DOI:10.1016/j.jormas.2025.102223

Yingxin Wang , Nana Liu , Xin Zhang , Min Dai , Nini Zhang , Guilin Huang

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Abstract

Objective

To investigate the reparative effect of hypoxia pretreated hAMSCs on radiation-induced damage to salivary gland function in mice.

Methods

hAMSCs were separated from human amniotic tissues by mechanical and enzymatic digestion methods and a 15 Gy electron beam was used to locally irradiate the neck of mouse to create a salivary gland injury model. The mouse models were randomly divided into four groups: control group, IR+PBS group, IR+Nor group and IR+HP group.

Result

Two months after hAMSCs injection, the saliva flow of mice in the IR+PBS group was significantly lower than that of the control group (P < 0.05). The saliva flow of mice in the IR+Nor group and IR+HP group were significantly increased compared to the IR+PBS group (P < 0.05). The cell apoptosis rate of the IR+PBS group was sensibly higher than that of the blank control group (P < 0.05). The cell apoptosis rates of the IR+Nor group and the IR+HP group were lower than that of the IR+PBS group. In addition, the apoptosis rate of the IR+HP group was lower than that of the IR+Nor group (P < 0.05). The changes of IOD of α-Amy in each group showed that the expression of α - Amy in the IR+PBS group was significantly lower than that in the blank control group (P < 0.05). Compared with the IR+PBS group, the IR+Nor group and the IR+HP group showed an obvious increase in the expression of α-Amy (P < 0.05).

Conclusion

Low oxygen pretreatment of hAMSCs could more effectively repair the function of radiation-induced salivary gland compared to normoxic cultivation.

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缺氧预处理人羊膜间充质干细胞修复辐射性唾液腺损伤功能的研究。

目的：研究缺氧预处理的 hAMSCs 对小鼠唾液腺功能辐射损伤的修复作用：方法：采用机械和酶解方法从人羊膜组织中分离出hAMSCs，用15 Gy电子束局部照射小鼠颈部，建立唾液腺损伤模型。小鼠模型随机分为四组：对照组、IR+PBS组、IR+Nor组和IR+HP组：结果：注射 hAMSCs 两个月后，IR+PBS 组小鼠的唾液流量明显低于对照组（PC 结论：IR+PBS 组小鼠的唾液流量明显低于对照组（PC）：与常氧培养相比，低氧预处理 hAMSCs 能更有效地修复辐射引起的唾液腺功能。

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