Future Challenge: Assessing the Antibiotic Susceptibility Patterns of Staphylococcus Species Isolated from Canine Otitis Externa Cases in Western Romania.

IF 4.3 2区 医学 Q1 INFECTIOUS DISEASES
János Dégi, Sorin Morariu, Florin Simiz, Viorel Herman, Florin Beteg, Diana Maria Dégi
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引用次数: 0

Abstract

Introduction: Antimicrobial resistance (AMR) has surfaced as a critical challenge to public health on a global scale. The precise and swift identification of resistance to antimicrobial agents, along with timely and suitable antimicrobial therapy paired with effective stewardship practices, is crucial for managing the rise and dissemination of antimicrobial resistance. The objective of our investigation was to outline the antimicrobial resistance profile of Staphylococcus spp., a significant contributor to canine otitis, a prevalent condition in dogs, isolated in Western Romania.

Methods and materials: All data were collected from clinical cases of canine otitis externa which presented at the University Clinic of the Faculty of Veterinary Medicine in Timișoara/Romania. A clinical evaluation was conducted, from which era swabs are usually collected and sent for analysis at the laboratory. Laboratory analysis included the microbiological examination for identifying Staphylococcus spp. and determining antibiotic susceptibility phenotypes. Statistical analysis was implemented on all data that were collected. The ear swabs were processed with standard procedures for cultivating and identifying bacteria. The resulting subcultures were processed to determine the staphylococcal species on the GP ID Cards of the Vitek® 2 automatic system. The antimicrobial susceptibility profiles were detected by the Vitek® 2 system using an AST-GP80 card. These isolated Staphylococcus spp. strains were further processed by real-time PCR and PCR-RFLP.

Results: Of all the auricular exudate samples analyzed, 76 were positive for Staphylococcus spp. (59.38%). Within these, in 82% of auricular samples, six distinct Staphylococcus spp. were identified (Staphylococcus (S.) pseudintermedius, S. intermedius, S. hyicus, S. delphiny, S. shleiferi, and S. aureus). Our data indicate that the PCR-RFLP assay is a practical approach to S. pseudintermedius identification, allowing for discrimination from the other Staphylococcus Intermedius Group (SIG) species and important staphylococcal pathogens of dogs. The highest frequency of resistant S. pseudintermedius isolates was detected against tetracycline (21/34; 61.76%; p-value 0.003), gentamicin (20/34; 58.82%), and kanamycin (20/34; 58.82%).

Conclusions: These results are essential to guide the prudent use of antibiotics in veterinary medicine. They will also help design efficient control strategies and measure their effectiveness.

未来的挑战:评估罗马尼亚西部犬外耳炎病例中分离的葡萄球菌的抗生素敏感性模式。
抗菌素耐药性(AMR)已成为全球范围内公共卫生面临的重大挑战。准确和迅速查明对抗菌素药物的耐药性,以及及时和适当的抗菌素治疗与有效的管理做法相结合,对于管理抗菌素耐药性的上升和传播至关重要。我们调查的目的是概述葡萄球菌的抗菌素耐药性概况,葡萄球菌是犬中耳炎的重要贡献者,犬中耳炎是犬的一种常见疾病,在罗马尼亚西部分离。方法和材料:所有数据收集自Timișoara/罗马尼亚兽医学院大学诊所的犬外耳炎临床病例。进行临床评估,通常从中收集时代拭子并送到实验室进行分析。实验室分析包括鉴定葡萄球菌的微生物学检查和确定抗生素敏感性表型。对收集到的所有数据进行统计分析。耳拭子按照培养和鉴定细菌的标准程序进行处理。在Vitek®2自动系统的GP ID卡上处理所得传代培养以确定葡萄球菌种类。通过使用AST-GP80卡的Vitek®2系统检测抗微生物药敏谱。对分离得到的葡萄球菌进行实时荧光定量PCR和PCR- rflp分析。结果:所有耳渗出液标本中,葡萄球菌阳性76份(59.38%)。其中,在82%的耳穴样本中鉴定出6种不同的葡萄球菌(假中间葡萄球菌、中间葡萄球菌、hyicus葡萄球菌、delphiny葡萄球菌、shleiferi葡萄球菌和金黄色葡萄球菌)。我们的数据表明,PCR-RFLP分析是一种实用的假中间葡萄球菌鉴定方法,可以与其他中间葡萄球菌群(SIG)和狗的重要葡萄球菌病原体进行区分。假中间链球菌对四环素的耐药频率最高(21/34;61.76%;p值0.003),庆大霉素(20/34;58.82%),卡那霉素(20/34;58.82%)。结论:这些结果对指导兽药抗菌药物的谨慎使用具有重要意义。它们还将有助于设计有效的控制战略并衡量其有效性。
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来源期刊
Antibiotics-Basel
Antibiotics-Basel Pharmacology, Toxicology and Pharmaceutics-General Pharmacology, Toxicology and Pharmaceutics
CiteScore
7.30
自引率
14.60%
发文量
1547
审稿时长
11 weeks
期刊介绍: Antibiotics (ISSN 2079-6382) is an open access, peer reviewed journal on all aspects of antibiotics. Antibiotics is a multi-disciplinary journal encompassing the general fields of biochemistry, chemistry, genetics, microbiology and pharmacology. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. Therefore, there is no restriction on the length of papers.
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