Study the effect of Lactobacillus plantarum ATCC 14917 for caries prevention and anti-obesity.

IF 4 2区 农林科学 Q2 NUTRITION & DIETETICS
Frontiers in Nutrition Pub Date : 2024-12-24 eCollection Date: 2024-01-01 DOI:10.3389/fnut.2024.1511660
Wei Yang, Mingxin Jiang, Bairu Chen, Kongzhao Jiang, Nan Ma, Yimin Li, Meng Wang, Meihua Bao, Chengyue Wang, Xiaopeng Yang
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引用次数: 0

Abstract

Introduction: A complicated scenario where "multiple disease threats coexist and multiple health influencing factors are intertwined" is demonstrated by the fact that dental caries, obesity myopia and scoliosis have emerged as global public health issues. The problem of diseases co-existing in living things can be resolved by using probiotics. Lactobacillus plantarum, has gained attention recently due to its probiotic properties, useful traits, and potential medical applications.

Objective: Examining the anti-obesity and anti-caries effects of L. plantarum ATCC 14917 on dental caries and obese rat models caused by a high-fat and high-sugar diet is the aim of this study.

Method: In vitro, we assessed the L. plantarum strain's probiotic properties, such as its antibacterial activity and ability to build biofilms, to determine its ability to inhibit Streptococcus mutans. Prior to the in vivo experiment, the subsist test for L. plantarum ATCC 14917 was carried out by mimicking its capacity to lower blood sugar and blood lipid levels as well as its tolerance to gastrointestinal disorders. In order to assess the health promotion effect of L. plantarum in vivo. Three-week-old rats were fed a high-sugar, high-fat diet for 8 weeks. They were split into three groups: the control group (Control), the caries and obesity group (CA _OB) and the caries and obesity treated with L. plantarum ATCC14917 group (LP). L. plantarum ATCC 14917 was applied during the experiment, and the associated indices were then thoroughly assessed. These included the use of Mirco-CT to calculate the enamel volume, the staining of liver and fat cell sections, serological analysis, and 16S rRNA sequencing of feces.

Results: It was proved that the L. plantarum could inhibit the proliferation of S. mutans and remove dental plaque biofilm in time, which showed the remarkable effects of anti-caries in vitro. The demineralization rate of enamel decreased by 44.10% due to the inhibition of acid production by pathogenic bacteria. Moreover, In intestinal and stomach juice simulations, L. plantarum has a high survival rate. The characteristics of bacterial activity in a wide range of pH could degrade triglycerides and glucose in vitro smoothly. The LP group demonstrated it by reducing animal weight, serum biochemical indices, and HE-stained adipocytes as compared to the CA_OB group. 16S rRNA sequencing data showed that a high-fat and high-sugar diet induced the imbalance of intestinal flora, which showed an increase in microbial abundance, including unclassified_o__Clostridia_UCG-014, unclassified_f__Oscillospiraceae, Turicibacter, unclassified_f__Lachnospiraceae, Clostridium_sensu_stricto_1. After the intervention of L. plantarum, the number of Lactobacillus, Limosilactobacillus, unclassified_f__Muribaculaceae, Blautia, Faecalibaculum increased significantly.

Conclusion: Therefore, L. plantarum ATCC 14917 performed the potential of reducing tooth decay and controlling weight gain by a single strain. Support the management of dental caries and obesity, and establish a foundation for future functional food research and development.

研究植物乳杆菌ATCC 14917预防龋齿和抗肥胖的作用。
摘要:龋齿、肥胖、近视和脊柱侧凸已成为全球性的公共卫生问题,呈现出“多种疾病威胁并存,多种健康影响因素相互交织”的复杂局面。利用益生菌可以解决生物体内疾病共存的问题。植物乳杆菌(Lactobacillus plantarum)近年来因其益生菌特性、有用特性和潜在的医学应用而备受关注。目的:探讨植物乳杆菌ATCC 14917对高脂高糖饮食致龋和肥胖大鼠模型的抗肥胖和抗龋作用。方法:在体外,我们评估了L. plantarum菌株的益生菌特性,如抗菌活性和构建生物膜的能力,以确定其抑制变形链球菌的能力。在体内实验之前,通过模拟L. plantarum ATCC 14917降低血糖和血脂水平的能力以及对胃肠道疾病的耐受性,对其进行了生存试验。为了评价植物乳杆菌在体内的健康促进作用。三周大的大鼠被喂食高糖、高脂肪的饮食8 周。将其分为对照组(control)、龋齿肥胖组(CA _OB)和植物乳杆菌ATCC14917治疗龋齿肥胖组(LP)。试验期间施用植物L. plantarum ATCC 14917,并对相关指标进行全面评价。这些包括使用micro - ct计算牙釉质体积,肝脏和脂肪细胞切片染色,血清学分析和粪便16S rRNA测序。结果:证明植物乳杆菌能抑制变形链球菌的增殖,及时去除牙菌斑生物膜,体外抗龋效果显著。病原菌对牙釉质产酸的抑制使牙釉质脱矿率降低了44.10%。此外,在肠道和胃液模拟中,植物乳杆菌具有较高的存活率。细菌在较宽pH范围内的活性特点能在体外顺利降解甘油三酯和葡萄糖。与CA_OB组相比,LP组通过降低动物体重、血清生化指标和he染色脂肪细胞来证明这一点。16S rRNA测序数据显示,高脂高糖饮食导致肠道菌群失衡,微生物丰度增加,包括unclassified_o_ clostridia_ucg -014、unclassified_f_ oscillospiraceae、Turicibacter、unclassified_f_ lachnospiraceae、Clostridium_sensu_stricto_1。植物乳杆菌干预后,乳酸菌、Limosilactobacillus、unclassified_f_muribaculaceae、Blautia、Faecalibaculum的数量显著增加。结论:单品系植物乳杆菌ATCC 14917具有减少蛀牙和控制体重增加的潜力。支持龋齿和肥胖的管理,为未来功能食品的研发奠定基础。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
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来源期刊
Frontiers in Nutrition
Frontiers in Nutrition Agricultural and Biological Sciences-Food Science
CiteScore
5.20
自引率
8.00%
发文量
2891
审稿时长
12 weeks
期刊介绍: No subject pertains more to human life than nutrition. The aim of Frontiers in Nutrition is to integrate major scientific disciplines in this vast field in order to address the most relevant and pertinent questions and developments. Our ambition is to create an integrated podium based on original research, clinical trials, and contemporary reviews to build a reputable knowledge forum in the domains of human health, dietary behaviors, agronomy & 21st century food science. Through the recognized open-access Frontiers platform we welcome manuscripts to our dedicated sections relating to different areas in the field of nutrition with a focus on human health. Specialty sections in Frontiers in Nutrition include, for example, Clinical Nutrition, Nutrition & Sustainable Diets, Nutrition and Food Science Technology, Nutrition Methodology, Sport & Exercise Nutrition, Food Chemistry, and Nutritional Immunology. Based on the publication of rigorous scientific research, we thrive to achieve a visible impact on the global nutrition agenda addressing the grand challenges of our time, including obesity, malnutrition, hunger, food waste, sustainability and consumer health.
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