A novel glucan from Sparassis latifolia: purification, structural characterization and protective effect on gastric mucosal cells against ethanol-induced damage
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引用次数: 0
Abstract
Sparassis latifolia is a rare edible mushroom with many pharmacological activities. Active screening found that the crude polysaccharide from this mushroom (SLP) exhibited noticeable gastroprotective effects. However, it remains unclear regarding the underlying regulatory mechanism of the gastroprotective effects of SLP. A novel polysaccharide SLP-2 was purified from the fruiting bodies of S. latifolia, and characterized using FT-IR, HPAEC, SEM, SEC–MALLS-RI, methylation, and NMR analysis. Structural characterization revealed that the backbone of SLP-2 was mainly composed of → 4)-α-D-Glcp-(1 → , → 3)-α-D-Glcp-(1 → and → 3,4)-α-D-Glcp-(1 → . The branched chain primarily consisted of β-D-Glcp-(1 → linked to the sugar residue → 3, 6)-β-D-Glcp-(1 → at the O-6 position and → 3,4)-β-D-Glcp-(1 → at the O-4 position. Physiological experimental results demonstrated that SLP-2 effectively protected GES-1 cells against ethanol-induced damage by suppressing intracellular ROS levels and cell apoptosis while elevating the activities of SOD and CAT enzymes. Furthermore, transcriptome analysis revealed molecular mechanisms underlying the gastroprotective effect of SLP-2, with KEGG enrichment suggesting associations with ferroptosis, TNF, PI3K–Akt, MAPK and IL-17 signaling pathways. These findings provide a technological foundation for valuable utilization of S. latifolia and emphasize the potential application of SLP-2 in repairing gastric mucosal injuries.
期刊介绍:
Chemical and Biological Technologies in Agriculture is an international, interdisciplinary, peer-reviewed forum for the advancement and application to all fields of agriculture of modern chemical, biochemical and molecular technologies. The scope of this journal includes chemical and biochemical processes aimed to increase sustainable agricultural and food production, the evaluation of quality and origin of raw primary products and their transformation into foods and chemicals, as well as environmental monitoring and remediation. Of special interest are the effects of chemical and biochemical technologies, also at the nano and supramolecular scale, on the relationships between soil, plants, microorganisms and their environment, with the help of modern bioinformatics. Another special focus is the use of modern bioorganic and biological chemistry to develop new technologies for plant nutrition and bio-stimulation, advancement of biorefineries from biomasses, safe and traceable food products, carbon storage in soil and plants and restoration of contaminated soils to agriculture.
This journal presents the first opportunity to bring together researchers from a wide number of disciplines within the agricultural chemical and biological sciences, from both industry and academia. The principle aim of Chemical and Biological Technologies in Agriculture is to allow the exchange of the most advanced chemical and biochemical knowledge to develop technologies which address one of the most pressing challenges of our times - sustaining a growing world population.
Chemical and Biological Technologies in Agriculture publishes original research articles, short letters and invited reviews. Articles from scientists in industry, academia as well as private research institutes, non-governmental and environmental organizations are encouraged.