Magnetic graphene-enhanced exonuclease III assisted amplification strategy driven carbon nanozyme for tri-mode detection of Escherichia coli O157:H7

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-01-06 DOI:10.1016/j.foodchem.2025.142776

Yuwei Ren , Lulu Cao , Zongyang Li , Xin Zhang , Hui Li , Rui Jiao , Xiaoyu Yu , Zhao Liang , Ganghui Li , Xiyang Xia , Na Ling , Danfeng Zhang , Jianghua Cheng , Yingwang Ye

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Abstract

Ultra-precision point-of-care detection of Escherichia coli O157:H7 in foods is an important issue. Here, the detection sensitivity was improved by a signal cascade amplification strategy synergised by exonuclease III assisted isothermal amplification and reverse magnetic strategy. The double-stranded DNA formed by the aptamer and the target DNA as a sensing switch, avoiding the complex process of specific nucleic acid extraction. Further, the signal display element is a green nanozyme synthesized by co-doping Cu and Cl elements with lignin as precursor, which has excellent peroxidase activity and fluorescence properties. The detection limits of fluorescence, colorimetric, and photothermal modes were 6.1, 8.4, and 9.7 cfu/mL, respectively. In order to enhance the precision of cascade signal amplification and the portability of quantitative detection, we built a neural network BP model installed in a smartphone by measuring multi-component data, which provides flexible and selectable application scenarios for the on-site detection of foodborne pathogens.

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磁性石墨烯增强外切酶III辅助扩增策略驱动碳纳米酶三模式检测大肠杆菌O157:H7

食品中大肠杆菌O157:H7的超精密即时检测是一个重要问题。在这里，通过外切酶III辅助等温扩增和反磁策略协同的信号级联扩增策略提高了检测灵敏度。由适体与靶DNA组成的双链DNA作为感应开关，避免了复杂的特异性核酸提取过程。此外，信号显示元件是以木质素为前体共掺杂Cu和Cl元素合成的绿色纳米酶，具有优异的过氧化物酶活性和荧光特性。荧光、比色、光热模式的检出限分别为6.1、8.4、9.7 cfu/mL。为了提高级联信号放大的精度和定量检测的便携性，我们通过测量多组分数据，构建了安装在智能手机上的神经网络BP模型，为食源性致病菌的现场检测提供了灵活、可选择的应用场景。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.