An electrochemical immunosensing of electrochemically modified carbon cloth electrode based on functionalized gold nanoparticle-Prussian blue for the detection of aflatoxin B1 in vegetable oil industry

IF 8.5 1区 农林科学 Q1 CHEMISTRY, APPLIED
Hai Chi , Xuefei Wen , Huan Li , Jie Tang , Xingzhong Zhang , Hongrui Chen
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引用次数: 0

Abstract

This study developed an electrochemical immunosensor for the detection of aflatoxin B1 (AFB1) in vegetable oil, based on an electrochemical modified carbon cloth (EMCC) electrode modified with a composite functional layer of cross-linked o-aminothiophenol functionalized AuNPs (o-ATP@AuNPs)/Prussian Blue (PB). The EMCC electrode substrate was prepared by modifying carbon cloth through electrochemical methods to increase its surface area, which allowed for the effective deposition of o-ATP@AuNPs/PB composite functional layer and improved the conductivity of the electrode material. The synergistic effect of o-ATP@AuNPs and PB significantly enhanced the sensitivity of the electrochemical sensor. Additionally, the AuS bond between L-Cysteine (L-Cys) and o-ATP@AuNPs improved the stability of the sensing interface. Under optimal conditions, the BSA/anti-AFB1/L-Cys/o-ATP@AuNPs/PB/EMCC sensor was able to detect AFB1 in the range of 0 to 20 ng mL−1 using square wave voltammetry (SWV), with a detection limit of 0.015 ng mL−1. The proposed sensor holds promise for future applications in the sensitive detection of AFB1 in vegetable oils.
基于功能化金纳米粒子-普鲁士蓝电化学修饰碳布电极的电化学免疫传感检测植物油工业中黄曲霉毒素B1
本研究基于交联邻氨基噻吩功能化AuNPs (o-ATP@AuNPs)/普鲁士蓝(PB)复合功能层修饰的电化学修饰碳布(EMCC)电极,开发了一种用于植物油中黄曲霉毒素B1 (AFB1)检测的电化学免疫传感器。通过电化学方法对碳布进行改性,增加其表面积,制备EMCC电极衬底,使o-ATP@AuNPs/PB复合功能层有效沉积,提高了电极材料的导电性。o-ATP@AuNPs与PB的协同作用显著提高了电化学传感器的灵敏度。此外,l -半胱氨酸(L-Cys)与o-ATP@AuNPs之间的AuS键提高了传感界面的稳定性。在最佳条件下,利用方波伏安法(SWV), BSA/anti-AFB1/L-Cys/o-ATP@AuNPs/PB/EMCC传感器能够检测到0 ~ 20 ng mL−1范围内的AFB1,检出限为0.015 ng mL−1。该传感器有望在植物油中AFB1的灵敏检测中应用。
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来源期刊
Food Chemistry
Food Chemistry 工程技术-食品科技
CiteScore
16.30
自引率
10.20%
发文量
3130
审稿时长
122 days
期刊介绍: Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.
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