Cu2-xSe@Bi2MoO6 nanozyme-based immunoassay for the colorimetric detection of walnut allergen in foods

IF 8.5 1区 农林科学 Q1 CHEMISTRY, APPLIED
ShaSha Wang, Lingjie Sheng, Yang Lu
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Abstract

In the present study, we developed a nanozyme-based direct competitive immunoassay to detect walnut allergen (Jug r 4) in foods. Walnut monoclonal antibody (mAb) and Cu2−xSe@Bi2MoO6 nanocomposites were generated to form a signal probe by electrostatic adsorption. The nanocomposites had high peroxidase-like activity and could be stored at room temperature. The linear range of the optimized assay was 137.35 ± 0.14 to 3526.0 ± 4.53 μg/L with a limit of detection of 81.63 ± 0.89 μg/L. This method was validated by detecting walnut protein in bread, biscuits, and milk samples, providing recovery rates of 70.63–127.04 %. The fast and sensitive assay was suitable for the rapid detection of walnut allergen in foods.

Abstract Image

在本研究中,我们开发了一种基于纳米酶的直接竞争免疫测定法来检测食品中的核桃过敏原(Jug r 4)。核桃单克隆抗体(mAb)与 Cu2-xSe@Bi2MoO6 纳米复合材料通过静电吸附形成信号探针。纳米复合材料具有很高的过氧化物酶样活性,并可在室温下保存。优化后的检测方法的线性范围为 137.35 ± 0.14 至 3526.0 ± 4.53 μg/L,检出限为 81.63 ± 0.89 μg/L。该方法通过检测面包、饼干和牛奶样品中的核桃蛋白进行了验证,回收率为 70.63-127.04 %。这种快速灵敏的检测方法适用于快速检测食品中的核桃过敏原。
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来源期刊
Food Chemistry
Food Chemistry 工程技术-食品科技
CiteScore
16.30
自引率
10.20%
发文量
3130
审稿时长
122 days
期刊介绍: Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.
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