Quantitative nuclear magnetic analysis of human milk oligosaccharides 2’-fucosyllactose and 3-fucosyllactose in complicated food matrices

IF 8.5 1区农林科学 Q1 CHEMISTRY, APPLIED

Food Chemistry Pub Date : 2025-01-07 DOI:10.1016/j.foodchem.2025.142821

Puchang An , Dongming Lan , Di Feng , Yihang Zhang , Hongmei An , Liting Zheng , Zhuying Wu , Daobing Wang , Qiding Zhong

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Abstract

Human milk oligosaccharides (HMOs) are the third most abundant solid component in breast milk, which is essential for the healthy growth of infants. 2′-fucosyllactose (2’-FL) and 3-fucosyllactose (3-FL) are the main components of HMOs. In this study, we developed and validated a quantitative nuclear magnetic resonance (qNMR) method for determining HMOs in different complex food matrices. The spectra of HMOs often overlap with impurities in the samples, which hampers quantification using the conventional integral method. We overcame this obstacle by building the Global Spectrum Deconvolution-area coefficients algorithm.

The method proved to be precise, yielding satisfactory results in terms of precision (RSD% 0.5–1.9), trueness (bias% 1.9–8.2), and recovery (90.5–106.6 %). The limit of quantification for 2’-FL was 0.10 mg/mL, and for 3-FL, it was 0.15 mg/mL. A comparison using Deming regression between the existing high-performance liquid chromatography method and the developed qNMR method for determining HMOs demonstrated the high accuracy of the qNMR method. The proposed method allows for high-throughput measurements of large-volume samples due to its simple and fast sample preparation. Therefore, the method is an essential tool for determining HMOs in various complex foods.

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复杂食品基质中人乳低聚糖2′-焦糖乳糖和3-焦糖乳糖的定量核磁分析

人乳寡糖（HMOs）是母乳中含量第三高的固体成分，对婴儿的健康成长至关重要。2 ' - focusyllactose （2 ' - fl）和3- focusyllactose （3- fl）是HMOs的主要成分。在这项研究中，我们建立并验证了一种定量核磁共振（qNMR）方法，用于测定不同复杂食品基质中的HMOs。HMOs的光谱经常与样品中的杂质重叠，妨碍了传统积分法的定量。我们通过构建全局频谱反卷积-面积系数算法克服了这一障碍。结果表明，该方法精密度（RSD% 0.5 ~ 1.9）、准确度（偏差% 1.9 ~ 8.2）、回收率（90.5 ~ 106.6 %）令人满意。2′-FL的定量限为0.10 mg/mL， 3′-FL的定量限为0.15 mg/mL。采用Deming回归对现有的高效液相色谱法和qNMR法测定HMOs进行了比较，结果表明qNMR法具有较高的准确性。由于样品制备简单、快速，该方法可用于大容量样品的高通量测量。因此，该方法是测定各种复杂食品中HMOs的重要工具。

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来源期刊

Food Chemistry 工程技术-食品科技

CiteScore

16.30

自引率

10.20%

发文量

3130

审稿时长

122 days

期刊介绍： Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.