Circular RNA WRNIP1 activates the PI3K-AKT and ERK1/2 signaling pathways by binding to miR-129-5p/IGF2 axis and facilitates ovarian follicle development in chickens

IF 3.8 1区农林科学 Q1 AGRICULTURE, DAIRY & ANIMAL SCIENCE

Poultry Science Pub Date : 2025-02-01 DOI:10.1016/j.psj.2024.104757

Xiyu Zhao , Yuanhang Wei , Xinyan Li , Xinghong Yu , Zhenyu Lei , Yao Zhang , Shunshun Han , Huadong Yin , Can Cui

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引用次数: 0

Abstract

The healthy and orderly development of ovarian follicles is the basic premise to maintain normal and continuous egg production of chickens. Emerging researches continue to reveal the crucial functions of circular RNAs (circRNAs) involved in follicle development. In the present study, a novel circular RNA WRNIP1 (circWRNIP1) with higher abundance in healthy follicles than in atretic follicles was identified, suggesting its important regulatory role in follicle selection and maturation. In this study, we demonstrate that circWRNIP1 promotes chicken ovarian follicle development by enhancing granulosa cell proliferation and inhibiting apoptosis. Furthermore, we validated the targeting relationships between circWRNIP1 and miR-129-5p, as well as between miR-129-5p and IGF2. Mechanistically, through co-transfection experiments, we show that circWRNIP1 activates the PI3K-AKT and ERK1/2 signaling pathways via the miR-129-5p/IGF2 axis. The present study provides new insights into the downstream molecular mechanism of circRNAs regulating follicle development, and is expected to provide reliable targets for the subsequent establishment of new molecular breeding strategies.

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环状RNA WRNIP1通过结合miR-129-5p/IGF2轴激活PI3K-AKT和ERK1/2信号通路，促进鸡卵泡发育。

卵泡的健康有序发育是维持鸡正常连续产蛋的基本前提。新兴研究不断揭示环状rna （circRNAs）参与卵泡发育的关键功能。在本研究中，发现了一种新的环状RNA WRNIP1 (circWRNIP1)，其在健康卵泡中的丰度高于闭锁卵泡，提示其在卵泡选择和成熟中具有重要的调节作用。在这项研究中，我们证明circWRNIP1通过增强颗粒细胞增殖和抑制细胞凋亡来促进鸡卵泡发育。此外，我们验证了circWRNIP1与miR-129-5p之间以及miR-129-5p与IGF2之间的靶向关系。在机制上，通过共转染实验，我们发现circWRNIP1通过miR-129-5p/IGF2轴激活PI3K-AKT和ERK1/2信号通路。本研究为circRNAs调控卵泡发育的下游分子机制提供了新的见解，并有望为后续建立新的分子育种策略提供可靠的靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Poultry Science 农林科学-奶制品与动物科学

CiteScore

7.60

自引率

15.90%

发文量

审稿时长

94 days

期刊介绍： First self-published in 1921, Poultry Science is an internationally renowned monthly journal, known as the authoritative source for a broad range of poultry information and high-caliber research. The journal plays a pivotal role in the dissemination of preeminent poultry-related knowledge across all disciplines. As of January 2020, Poultry Science will become an Open Access journal with no subscription charges, meaning authors who publish here can make their research immediately, permanently, and freely accessible worldwide while retaining copyright to their work. Papers submitted for publication after October 1, 2019 will be published as Open Access papers. An international journal, Poultry Science publishes original papers, research notes, symposium papers, and reviews of basic science as applied to poultry. This authoritative source of poultry information is consistently ranked by ISI Impact Factor as one of the top 10 agriculture, dairy and animal science journals to deliver high-caliber research. Currently it is the highest-ranked (by Impact Factor and Eigenfactor) journal dedicated to publishing poultry research. Subject areas include breeding, genetics, education, production, management, environment, health, behavior, welfare, immunology, molecular biology, metabolism, nutrition, physiology, reproduction, processing, and products.