Differences in the composition of plasma metabolites and intestinal flora of piglets with different weaning weights revealed by untargeted metabolomics and 16S rRNA gene sequencing

IF 3.5 2区农林科学 Q1 AGRICULTURE, MULTIDISCIPLINARY

Journal of the Science of Food and Agriculture Pub Date : 2025-01-05 DOI:10.1002/jsfa.14114

Yanchao Geng, Long Li, Xuefeng Yuan, Liang Hong, Lei Pu, Shunyi Qin, Liuan Li, Hua Yang, Jianbin Zhang

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引用次数: 0

Abstract

BACKGROUND

Piglets with different weaning body weights exhibit varying growth performance. This study explores the relationship between their plasma metabolites and gut microbiota to reveal differences in metabolic regulation and microbial composition.

RESULTS

Plasma and colon content samples from piglets of different weaning weights were collected. Untargeted metabolomics, 16S rRNA gene sequencing, multivariate statistics, and bioinformatics were used to identify and compare metabolites.

Six key findings emerged. First, 23 differential metabolites were found, with three upregulated in high-weight piglets and 20 downregulated in low-weight piglets. A total of 15 were lipids or lipid-like molecules. Second, metabolic pathway enrichment analysis indicated that the sphingolipid signaling pathway, HIF-1 signaling pathway, sphingolipid metabolism pathway, ascorbate and aldarate metabolism pathway, and glycine, serine, and threonine metabolism pathway were the most significantly affected pathways in the plasma of piglets with different weaning body weights. Third, alpha diversity was lower in low-weight weaned piglets. Fourth, Lactobacillus was 23.16% in high-weight piglets, higher than 19.62% in low-weight ones. Fifth, linear discriminant analysis effect size (LEfSe) analysis showed that Faecalibacterium is a biomarker for low-body-weight piglets and Oscillospira is a biomarker for high-body-weight piglets. Finally, Spearman correlation analysis indicated that Lactobacillus, Prevotella, Ruminococcus, and Oscillospira were negatively correlated with differential metabolites in plasma.

CONCLUSION

The plasma metabolites and colon microbiota differed between piglets of different body weights. Lipid-related plasma metabolites contributed to weight variation, being lower in heavier piglets. The colonic microbiota, especially Oscillospira and Roseburia, exhibited strong correlations with these metabolites. © 2025 Society of Chemical Industry.

查看原文本刊更多论文

非靶向代谢组学和16S rRNA基因测序揭示了不同断奶体重仔猪血浆代谢物组成和肠道菌群的差异。

背景：不同断奶体重的仔猪表现出不同的生长性能。本研究探讨了它们的血浆代谢物与肠道微生物群之间的关系，以揭示代谢调节和微生物组成的差异。结果：收集了不同断奶体重仔猪的血浆和结肠内容物样本。非靶向代谢组学、16S rRNA基因测序、多变量统计和生物信息学用于鉴定和比较代谢物。六个主要发现浮出水面。首先，发现了23种差异代谢物，其中3种在高重仔猪中上调，20种在低重仔猪中下调。共有15种是脂质或类脂分子。代谢途径富集分析表明，不同断奶体重仔猪血浆中鞘脂信号通路、HIF-1信号通路、鞘脂代谢途径、抗坏血酸和醛酸盐代谢途径以及甘氨酸、丝氨酸和苏氨酸代谢途径受影响最为显著。第三，低重断奶仔猪α多样性较低。4 .高重仔猪乳酸菌含量为23.16%，高于低重仔猪19.62%。第五，线性判别分析效应量（LEfSe）分析表明，Faecalibacterium是低体重仔猪的生物标志物，Oscillospira是高体重仔猪的生物标志物。最后，Spearman相关分析表明，乳酸杆菌、普雷沃氏菌、瘤胃球菌和Oscillospira与血浆中差异代谢物呈负相关。结论：不同体重仔猪血浆代谢物和结肠微生物群存在差异。与脂质相关的血浆代谢物有助于体重变化，在较重的仔猪中较低。结肠微生物群，尤其是Oscillospira和Roseburia，与这些代谢物表现出很强的相关性。©2025化学工业协会。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of the Science of Food and Agriculture 工程技术-农业综合

CiteScore

8.10

自引率

4.90%

发文量

634

审稿时长

3.1 months

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