Preparation, modification, characterization, and stability evaluation of 5-methyltetrahydrofolate liposomes

IF 8.5 1区 农林科学 Q1 CHEMISTRY, APPLIED
Ziyi Meng , Xu Yan , Nima Azarakhsh , Hanying Duan , Hosahalli S. Ramaswamy , Chao Wang
{"title":"Preparation, modification, characterization, and stability evaluation of 5-methyltetrahydrofolate liposomes","authors":"Ziyi Meng ,&nbsp;Xu Yan ,&nbsp;Nima Azarakhsh ,&nbsp;Hanying Duan ,&nbsp;Hosahalli S. Ramaswamy ,&nbsp;Chao Wang","doi":"10.1016/j.foodchem.2025.142792","DOIUrl":null,"url":null,"abstract":"<div><div>As an essential B vitamin, folate participates in one‑carbon metabolism. The 5-methyltetrahydrofolate (5-MTHF) avoids the drawbacks associated with folic acid and native folylpolyglutamate folate in food, thereby emerging as a superior alternative to folate supplement. To enhance the stability and digestibility of 5-MTHF, nanoliposome (NL) was modified using a layer-by-layer self-assembly method with chitosan (CH) and pectin (P). Chitosan–nanoliposome (CH–NL) and pectin–chitosan–nanoliposome (P–CH–NL) were created, each featuring a core–shell structure. P–CH–NL achieved an encapsulation efficiency of 64.62 %, loading efficiency of 1.05 mg/g, and particle size of 285.86 nm. It exhibited better physical stability and 5-MTHF retention (&gt;80 %) under various conditions, including salt and pH variations, as well as oxidative, thermal, fermentation, and UV stress. During <em>in vitro</em> digestion, P–CH–NL protected 5-MTHF until it was released into the small intestine. This study highlighted the application prospects of multilayer liposome-loaded 5-MTHF as a stable, highly digestible folate supplement.</div></div>","PeriodicalId":318,"journal":{"name":"Food Chemistry","volume":"471 ","pages":"Article 142792"},"PeriodicalIF":8.5000,"publicationDate":"2025-01-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Food Chemistry","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0308814625000421","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, APPLIED","Score":null,"Total":0}
引用次数: 0

Abstract

As an essential B vitamin, folate participates in one‑carbon metabolism. The 5-methyltetrahydrofolate (5-MTHF) avoids the drawbacks associated with folic acid and native folylpolyglutamate folate in food, thereby emerging as a superior alternative to folate supplement. To enhance the stability and digestibility of 5-MTHF, nanoliposome (NL) was modified using a layer-by-layer self-assembly method with chitosan (CH) and pectin (P). Chitosan–nanoliposome (CH–NL) and pectin–chitosan–nanoliposome (P–CH–NL) were created, each featuring a core–shell structure. P–CH–NL achieved an encapsulation efficiency of 64.62 %, loading efficiency of 1.05 mg/g, and particle size of 285.86 nm. It exhibited better physical stability and 5-MTHF retention (>80 %) under various conditions, including salt and pH variations, as well as oxidative, thermal, fermentation, and UV stress. During in vitro digestion, P–CH–NL protected 5-MTHF until it was released into the small intestine. This study highlighted the application prospects of multilayer liposome-loaded 5-MTHF as a stable, highly digestible folate supplement.
5-甲基四氢叶酸脂质体的制备、修饰、表征和稳定性评价
作为一种必需的B族维生素,叶酸参与一碳代谢。5-甲基四氢叶酸(5-MTHF)避免了与叶酸和天然叶酸聚谷氨酸叶酸相关的缺点,因此成为叶酸补充剂的优良替代品。为了提高5-MTHF的稳定性和可消化性,采用壳聚糖(CH)和果胶(P)层层自组装的方法对纳米脂质体(NL)进行了修饰,制备了壳聚糖-纳米脂质体(CH - NL)和果胶-壳聚糖-纳米脂质体(P - CH - NL),各具有核壳结构。P-CH-NL包封率为64.62 %,负载效率为1.05 mg/g,粒径为285.86 nm。在各种条件下,包括盐和pH变化,以及氧化、热、发酵和紫外线胁迫下,它表现出更好的物理稳定性和5-MTHF保留率(>80 %)。在体外消化过程中,P-CH-NL保护5-MTHF,直到其释放到小肠中。本研究强调了多层脂质体负载5-MTHF作为一种稳定、高消化的叶酸补充剂的应用前景。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
求助全文
约1分钟内获得全文 求助全文
来源期刊
Food Chemistry
Food Chemistry 工程技术-食品科技
CiteScore
16.30
自引率
10.20%
发文量
3130
审稿时长
122 days
期刊介绍: Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.
文献相关原料
公司名称
产品信息
阿拉丁
Soybean lecithin
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信