Combination of Lycium barbarum polysaccharide and chlorogenic acid: Characterization of solution property, interaction, and regulatory function on typical gut Bacteroides and pathogens

IF 8.5 1区 农林科学 Q1 CHEMISTRY, APPLIED
Lisi Liang , Lianzhu Lin , Mouming Zhao
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Abstract

Lycium barbarum polysaccharide (LBP) is a prebiotic that promotes the proliferation of beneficial bacteria, but lacks of regulatory function on harmful bacteria. In this study, chlorogenic acid (CGA) was used to achieve the functional enhancement of two LBPs (LBP-A and LBP-M). The combination of CGA resulted in changes in the solution properties of LBPs, manifested as increased pseudoplasticity, viscosity, turbidity, and decreased water mobility, absolute potential value, pH value. CGA was non-covalently bound to LBPs through hydrophobic interaction and hydrogen bond. LBP-A had strong affinity with CGA, mainly through hydrophobic interaction, while LBP-M had weak affinity with CGA, mainly through hydrogen bond. Both LBP-CGA complexes exhibited Bacteroides proliferative activity and gut pathogens inhibitory activity, among which LBP-A-CGA complex showed strong inhibitory effects on Escherichia coli and Bacillus cereus, and LBP-M-CGA complex showed strong inhibitory effect on Staphylococcus aureus. This study provided guidance for the development of LBP-CGA as novel prebiotic.

Abstract Image

Abstract Image

枸杞多糖与绿原酸的组合:溶液性质、相互作用及其对典型肠道拟杆菌和病原体的调节作用
枸杞多糖(LBP)是一种促进有益菌增殖的益生元,对有害菌缺乏调节作用。本研究采用绿原酸(chlorogenic acid, CGA)对两种lbp (LBP-A和LBP-M)进行功能增强。CGA的联合作用导致lbp的溶液性质发生变化,表现为假塑性、粘度、浊度增加,水迁移率、绝对电位值、pH值降低。CGA通过疏水相互作用和氢键与lbp非共价结合。LBP-A与CGA的亲和力较强,主要通过疏水作用,而LBP-M与CGA的亲和力较弱,主要通过氢键作用。LBP-CGA复合物均表现出拟杆菌的增殖活性和肠道病原菌的抑制活性,其中LBP-A-CGA复合物对大肠杆菌和蜡样芽孢杆菌有较强的抑制作用,LBP-M-CGA复合物对金黄色葡萄球菌有较强的抑制作用。本研究为LBP-CGA作为新型益生元的开发提供了指导。
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来源期刊
Food Chemistry
Food Chemistry 工程技术-食品科技
CiteScore
16.30
自引率
10.20%
发文量
3130
审稿时长
122 days
期刊介绍: Food Chemistry publishes original research papers dealing with the advancement of the chemistry and biochemistry of foods or the analytical methods/ approach used. All papers should focus on the novelty of the research carried out.
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阿拉丁
Chlorogenic acid
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